HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Engelhorn, M. Articles by Roux, L. Search for Related Content PubMed PubMed Citation Articles by Engelhorn, M. Articles by Roux, L. Agricola Articles by Engelhorn, M. Articles by Roux, L.

Molecular cloning and characterization of a Sendai virus internal deletion defective RNA

Department of Genetics and Microbiology, University of Geneva Medical School, C.M.U., 9 Avenue de Champel, 1211 Geneva 4, Switzerland

A small defective Sendai virus RNA was selectively amplified from a virus preparation obtained after serial undiluted passages in embryonated eggs. Preliminary characterization showed that this defective RNA was a true internal deletion defective RNA, containing the 5' and 3' ends of the non-defective viral genomic RNA. Cloning of this RNA after reverse transcription and polymerase chain reaction amplification was performed in such a way that an exact copy of the defective RNA could be obtained by transcription of the plasmid with T7 RNA polymerase. Sequence analysis of the plasmid allowed further characterization of the defective RNA. It was shown potentially to encode a C-terminally truncated nucleocapsid (NP) protein of 162 amino acids. This truncated NP protein was identified in cells naturally infected with the defective virus preparation. Moreover the protein produced was shown to correspond to the protein synthesized in vitro from the T7 polymerase transcript of the cloned defective genome.

Received 6 July 1992; accepted 8 September 1992.

This article has been cited by other articles:

				G. Mottet, V. Müller, and L. Roux Characterization of Sendai virus M protein mutants that can partially interfere with virus particle production J. Gen. Virol., November 1, 1999; 80(11): 2977 - 2986. [Abstract] [Full Text]

				S. Finke and K.-K. Conzelmann Virus Promoters Determine Interference by Defective RNAs: Selective Amplification of Mini-RNA Vectors and Rescue from cDNA by a 3' Copy-Back Ambisense Rabies Virus J. Virol., May 1, 1999; 73(5): 3818 - 3825. [Abstract] [Full Text]

				C. Tapparel, D. Maurice, and L. Roux The Activity of Sendai Virus Genomic and Antigenomic Promoters Requires a Second Element Past the Leader Template Regions: a Motif (GNNNNN)3 Is Essential for Replication J. Virol., April 1, 1998; 72(4): 3117 - 3128. [Abstract] [Full Text] [PDF]

				D. Kolakofsky, T. Pelet, D. Garcin, S. Hausmann, J. Curran, and L. Roux Paramyxovirus RNA Synthesis and the Requirement for Hexamer Genome Length: the Rule of Six Revisited J. Virol., February 1, 1998; 72(2): 891 - 899. [Full Text] [PDF]