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J Gen Virol 74 (1993), 39-46; DOI 10.1099/0022-1317-74-1-39
© 1993 Society for General Microbiology
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Importance of 3' non-coding sequences for efficient retrovirus-mediated gene transfer in avian cells revealed by self-inactivating vectors

Frédéric Flamant1, Denise Aubert1, Claude Legrand1, Francois-Loïc Cosset2 and Jacques Samarut1

1 Laboratoire de Biologie Moléculaire et Cellulaire, INRA-CNRS UMR 49, Ecole Normale Supérieure de Lyon, 46 allée d'Italie, 69364 Lyon Cedex 07
and2 Laboratoire de Biologie Cellulaire, Université Claude Bernard Lyon I, 43 boulevard du 11 novembre 1918, 69622 Villeurbanne Cedex, France

Avian leukosis virus-derived vectors were constructed with an internal transcriptional promoter and various 3' non-coding sequences. Deletions were introduced into the downstream U3 long terminal repeat (LTR) to obtain self-inactivation of LTR-mediated transcription after one round of replication. However, 3' non-coding sequences appeared to determine not only self-inactivation of the vectors but also gene transfer efficiency. Further analysis revealed the influence of these sequences on both internal gene expression and RNA packaging. One construct permitted gene transfer while inactivating 5' LTR-promoted transcription.

Received 8 June 1992; accepted 8 September 1992.


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