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J Gen Virol 74 (1993), 99-102; DOI 10.1099/0022-1317-74-1-99
© 1993 Society for General Microbiology
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Expression of Bombyx mori cytoplasmic polyhedrosis virus polyhedrin in insect cells by using a baculovirus expression vector, and its assembly into polyhedra

Hajime Mori1, Reiko Ito1, Hiroshi Nakazawa1, Motoyuki Sumida1, Fujiyoshi Matsubara1 and Yuzo Minobe2

1 Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology, Kyoto 606
and2 National Institute of Agrobiological Resources, Tsukuba 305, Japan

A cDNA encoding the cytoplasmic polyhedrin of Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) strain H was introduced into an improved baculovirus expression vector which can be utilized to express foreign genes in the Spodoptera frugiperda cell line IPLB-SF-21AE (Sf21 cells) and the B. mori cell line BmN. A recombinant virus produced large, cubic inclusion body-like structures in infected Sf21 and BmN cells. Western blot analysis showed that these structures were BmCPV polyhedra. This result suggested that the supramolecular assembly of BmCPV polyhedrin is responsible for its properties.

Received 17 June 1992; accepted 21 September 1992.


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Copyright © 1993 by the Society for General Microbiology.