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J Gen Virol 74 (1993), 2163-2170; DOI 10.1099/0022-1317-74-10-2163
© 1993 Society for General Microbiology
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Latency and reactivation of Marek's disease virus in B lymphocytes transformed by avian leukosis virus

Ellen F. Fynan1,{dagger}, Donald L. Ewert2 and Timothy M. Block1

1 Jefferson Cancer Institute and the Department of Microbiology and Immunology, Thomas Jefferson Medical College, Philadelphia, Pennsylvania 19107
and2 The Wistar Institute, Philadelphia, Pennsylvania 19104, U.S.A.

The physical and biological state of the Marek's disease virus (MDV) genome in avian leukosis virus (ALV)-transformed cells is characterized using cell lines established from ALV tumours co-infected with the SB-1 strain of MDV. The MDV genome within the ALV-transformed cells was found to be methylated at 5' CpG 3' dinucleotides. Less than 2% of the tumour cells expressed MDV antigen and only one virus plaque that was characteristic of an MDV infection was noted when tumour cells were cocultured with fibroblasts permissive for a productive MDV infection. However, when methylation of the MDV genome was prevented by culturing the tumour cell lines in the presence of 5-azacytidine, both MDV antigen expression and viral replication increased. Based on these results, it appears that MDV resides within the ALV-transformed cells in a latent state and that MDV latency might be influenced, to some extent, by methylation of the MDV genome.

{dagger} Present address: Department of Pathology, S2-146, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655, U.S.A.

Received 4 January 1993; accepted 14 June 1993.


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