| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Virology, Central Veterinary Institute, Houtribweg 39, P.O. Box 365, NL-8200 AB Lelystad, The Netherlands
The virulence, pathogenicity and immunogenicity of two pseudorabies virus (PRV) variants were investigated in 3-week-old pigs that had been intranasally infected. Variant M303 (
125,126) lacked amino acids valine (125) and cysteine(126) in an immunodominant antigenic region of glycoprotein I (gI) containing two discontinuous antigenic domains, whereas M304 (59,60) lacked amino acids glycine(59) and aspartic acid(60) in a continuous antigenic domain. M303 (125,126) was not virulent for pigs, but M304 (59,60) was as virulent as wild-type PRV: all pigs died within 8 days of infection. Both gI mutant viruses replicated in the oropharyngeal mucosa, although M304 (59,60) replicated to higher virus titres than M303 (125,126), and virus was recovered from various tissues. However, in contrast to M304 (59,60), M303 (125,126) was not recovered from any central nervous system (CNS) tissues examined. Thus, the tendency of PRV to locate in the CNS was markedly reduced by deleting amino acids valine(125) and cysteine(126) of gI. Pigs immunized with M303 were completely protected against challenge infection; no clinical signs of disease were detected, no virus was shed, and no secondary antibody response was detected. Thus, deleting amino acids valine(125) and cysteine(126) in gI decreases virulence and neurotropism and does not affect immunogenicity.
Received 20 March 1993;
accepted 18 May 1993.
This article has been cited by other articles:
|
|
 |
|
  B. Muylkens, F. Meurens, F. Schynts, F. Farnir, A. Pourchet, M. Bardiau, S. Gogev, J. Thiry, A. Cuisenaire, A. Vanderplasschen, et al. Intraspecific bovine herpesvirus 1 recombinants carrying glycoprotein E deletion as a vaccine marker are virulent in cattle. J. Gen. Virol., August 1, 2006; 87(Pt 8): 2149 - 2154. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Paulus, P. J. Sollars, G. E. Pickard, and L. W. Enquist Transcriptome Signature of Virulent and Attenuated Pseudorabies Virus-Infected Rodent Brain J. Virol., February 15, 2006; 80(4): 1773 - 1786. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Al-Mubarak, Y. Zhou, and S. I. Chowdhury A Glycine-Rich Bovine Herpesvirus 5 (BHV-5) gE-Specific Epitope within the Ectodomain Is Important for BHV-5 Neurovirulence J. Virol., May 1, 2004; 78(9): 4806 - 4816. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Flamand, T. Bennardo, N. Babic, B. G. Klupp, and T. C. Mettenleiter The Absence of Glycoprotein gL, but Not gC or gK, Severely Impairs Pseudorabies Virus Neuroinvasiveness J. Virol., November 15, 2001; 75(22): 11137 - 11145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Johnson, M. Webb, T. W. Wisner, and C. Brunetti Herpes Simplex Virus gE/gI Sorts Nascent Virions to Epithelial Cell Junctions, Promoting Virus Spread J. Virol., January 15, 2001; 75(2): 821 - 833. [Abstract] [Full Text]
|
|
|
|
|
|
R. S. Tirabassi and L. W. Enquist Role of the Pseudorabies Virus gI Cytoplasmic Domain in Neuroinvasion, Virulence, and Posttranslational N-Linked Glycosylation J. Virol., April 15, 2000; 74(8): 3505 - 3516. [Abstract] [Full Text]
|
|
|
|
|
|
S. I. Chowdhury, B. J. Lee, A. Ozkul, and M. L. Weiss Bovine Herpesvirus 5 Glycoprotein E Is Important for Neuroinvasiveness and Neurovirulence in the Olfactory Pathway of the Rabbit J. Virol., March 1, 2000; 74(5): 2094 - 2106. [Abstract] [Full Text]
|
|
|
|
|
|
M. J. Masse, A. Jöns, J. M. Dijkstra, T. C. Mettenleiter, and A. Flamand Glycoproteins gM and gN of Pseudorabies Virus Are Dispensable for Viral Penetration and Propagation in the Nervous Systems of Adult Mice J. Virol., December 1, 1999; 73(12): 10503 - 10507. [Abstract] [Full Text]
|
|
|
|
|
|
M. Yang, J. P. Card, R. S. Tirabassi, R. R. Miselis, and L. W. Enquist Retrograde, Transneuronal Spread of Pseudorabies Virus in Defined Neuronal Circuitry of the Rat Brain Is Facilitated by gE Mutations That Reduce Virulence J. Virol., May 1, 1999; 73(5): 4350 - 4359. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
