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Isolation of a retrovirus from two fish cell lines developed from chinook salmon (Oncorhynchus tshawytscha) with plasmacytoid leukaemia

¹ Malaspina University College, 900 Fifth Street, Nanaimo, British Columbia V9R 5S5
and² Department of Fisheries and Oceans, Pacific Biological Station, Nanaimo, British Columbia V9R 5K6, Canada

Two new cell lines developed from chinook salmon with plasmacytoid leukaemia have been found to be producing a virus. The virus has been identified as a retrovirus based on: type of c.p.e. induced in culture; morphology and density of the particle; presence of Mn²⁺-dependent, poly(rA)-directed reverse transcriptase activity which was associated with a density of 1·16 to 1·18 g/ml in sucrose; electrophoretic pattern of the polypeptides from purified virions; elevated [³H]UTP labelling of RNA in the cell cultures occurring at a density of 1·16 to 1·18 g/ml in sucrose. This report describes the first isolation of a retrovirus from a salmonid cell line.

Received 18 November 1992; accepted 14 May 1993.