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Effect of the alfalfa mosaic virus movement protein expressed in transgenic plants on the permeability of plasmodesmata

¹ Institut de Biologie Moléculaire des Plantes, CNRS, Université Louis Pasteur, 12 rue du Général Zimmer, 67084 Strasbourg-Cedex, France
and² Department of Cell Biology, John Innes Institute, Colney Lane, Norwich NR4 7UH, U.K.

Symplastic transport of different sized fluorescent probes has been assessed in leaf epidermal cells of transgenic Nicotiana plants expressing the movement protein (MP) of alfalfa mosaic virus (AMV). In both N. tabacum and N. benthamiana, the size exclusion limit (SEL) of plasmodesmata increased from M_r 1000, which represents the commonly accepted limit, to over 4.4K. However, in control plants, movement of a 3K probe was seen in 11 to 22% of the injections, indicating that plasmodesmata may on occasion allow the passage of molecules larger than was previously thought. The increase of SEL due to the presence of the AMV MP, although significant, remains insufficient to permit the passage of viral particles and the possibility of other mechanisms involved in viral cell-to-cell spread is discussed.

Received 30 March 1993; accepted 15 June 1993.

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