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J Gen Virol 74 (1993), 2463-2468; DOI 10.1099/0022-1317-74-11-2463
© 1993 Society for General Microbiology
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Complete nucleotide sequence and coding strategy of rice hoja blanca virus RNA4

Bertha-Cecilia Ramirez1, Ivan Lozano2, Luis-Miguel Constantino2, Anne-Lise Haenni1 and Lee A. Calvert2

1 Institut Jacques Monod, 2 Place Jussieu-Tour 43, 75251 Paris Cedex 05, France
and2 Centro Internacional de Agricultura Tropical (CIAT), A.A. 6713, Cali, Colombia

The complete sequence of rice hoja blanca virus (RHBV) RNA4 has been determined, based on the sequence of the corresponding cDNA clones. RNA4 consists of 1991 nucleotides with two open reading frames (ORFs). One putative ORF is located in the 5'-proximal region of the viral RNA4; it encodes a protein of predicted Mr 20076 which corresponds to the major non-structural protein that accumulates in RHBV-infected rice plants, and which bears limited sequence identity with the helper component of tobacco vein mottling potyvirus. The other ORF is located in the 5'-proximal region of the viral complementary RNA4 and encodes a protein of predicted Mr 32469. Between the two ORFs is an intergenic region of 524 nucleotides, part of which can theoretically adopt a stable stem-loop structure; the 5' and 3' ends can potentially base-pair over 16 nucleotides, producing a pan-handle configuration. These characteristics are in favour of an ambisense coding strategy for RHBV RNA4.

Received 2 July 1993; accepted 9 July 1993.


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