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Reasons for the low accumulation level of aphid transmission factor protein in infected leaves with an aphid-non-transmissible cauliflower mosaic virus isolate, CM1841

¹ Laboratory of Plant Pathology, Faculty of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-01, Japan
² Laboratory of Plant Pathology, Faculty of Agriculture, Kyoto Prefectural University, Sakyo-ku, Kyoto 606
and³ Department of Microbiology, Kyoto Prefectural University of Medicine, Kamikyo-ku, Kyoto 602, Japan

The synthesis and accumulation of aphid transmission factor protein (p18) in cauliflower mosaic virus (CaMV)-infected turnip protoplasts were examined in time course and pulse-labelling experiments, comparing an aphid-non-transmissible isolate (CM1841) with an in vitro recombinant aphid-transmissible CaMV (CMBX) generated from the CM1841 isolate. There was little difference in the synthesis and accumulation of p18 between CM1841- and CMBX-infected protoplasts. When the accumulation of p18 in infected leaves was monitored from 3 to 28 days post-symptom emergence (p.e.) by Western blotting, the amount of p18 accumulated in CM1841-infected leaves continuously decreased from 3 days p.e. throughout the experimental period, whereas the amount of p18 in CMBX-infected leaves was lowest at 3 days p.e. and increased thereafter. These results suggested that CM1841 differed from CMBX not in the synthesis of p18 but in the stability of p18 in infected leaves.

Received 26 April 1993; accepted 2 July 1993.

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