HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Poisson, F. Articles by Goudeau, A. Search for Related Content PubMed PubMed Citation Articles by Poisson, F. Articles by Goudeau, A. Agricola Articles by Poisson, F. Articles by Goudeau, A.

Characterization of RNA-binding domains of hepatitis delta antigen

¹ Unité de Virologie, Département de Microbiologie Médicale et Moléculaire, URA CNRS 1334, CHU Bretonneau, 2 Boulevard Tonnellé, 37044 Tours Cedex, France
² Research and Development SORIN Biomedica S.p.A., Saluggia 13040
and³ Department of Genetics, General and Molecular Biology, University of Naples, Napoli 8100, Italy

Hepatitis delta antigen (HDAg), the only protein encoded by the hepatitis delta virus (HDV), binds specifically genomic and antigenomic strands of the HDV RNA. In a previous study, three recombinant HDAg subdomains were synthesized, covering residues 11 to 78, 79 to 163 and 164 to 212, and only the middle domain was shown to be responsible for the binding to HDV RNA. To investigate HDAg sequences involved in HDV RNA binding, we synthesized five peptides, 15 to 29 residues in length, and tested their ability to bind HDV RNA using a simple non-radioactive ELISA with digoxigenin-labelled HDV genomic or antigenomic RNA probes. The specificity of interactions was demonstrated by comparison with control peptides and non-HDV RNA probes, and with an inhibition assay using recombinant HDAg. The HDAg-binding domain found within the middle region (79 to 163) of HDAg was more finely mapped: it is located between residues 79 and 107. In addition, another domain (residues 2 to 27) of HDAg was also found to bind specifically to HDV RNA. These two peptides share sequence similarities at residues 2 to 10 and 97 to 107 with other RNA-binding domains.

Received 19 March 1993; accepted 10 June 1993.

This article has been cited by other articles:

				C.-C. Wang, T.-C. Chang, C.-W. Lin, H.-L. Tsui, P. B. C. Chu, B.-S. Chen, Z.-S. Huang, and H.-N. Wu Nucleic acid binding properties of the nucleic acid chaperone domain of hepatitis delta antigen Nucleic Acids Res., November 15, 2003; 31(22): 6481 - 6492. [Abstract] [Full Text] [PDF]

				Q. Cheng, G. C. Jayan, and J. L. Casey Differential Inhibition of RNA Editing in Hepatitis Delta Virus Genotype III by the Short and Long Forms of Hepatitis Delta Antigen J. Virol., July 15, 2003; 77(14): 7786 - 7795. [Abstract] [Full Text] [PDF]

				Z.-S. Huang, W.-H. Su, J.-L. Wang, and H.-N. Wu Selective Strand Annealing and Selective Strand Exchange Promoted by the N-terminal Domain of Hepatitis Delta Antigen J. Biol. Chem., February 14, 2003; 278(8): 5685 - 5693. [Abstract] [Full Text] [PDF]

				S. Sato, S. K. Wong, and D. W. Lazinski Hepatitis Delta Virus Minimal Substrates Competent for Editing by ADAR1 and ADAR2 J. Virol., September 15, 2001; 75(18): 8547 - 8555. [Abstract] [Full Text] [PDF]

				Z.-S. Huang and H.-N. Wu Identification and Characterization of the RNA Chaperone Activity of Hepatitis Delta Antigen Peptides J. Biol. Chem., October 9, 1998; 273(41): 26455 - 26461. [Abstract] [Full Text] [PDF]

				H.-C. Chou, T.-Y. Hsieh, G.-T. Sheu, and M. M. C. Lai Hepatitis Delta Antigen Mediates the Nuclear Import of Hepatitis Delta Virus RNA J. Virol., May 1, 1998; 72(5): 3684 - 3690. [Abstract] [Full Text] [PDF]