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Murine cytotoxic T cell response specific for human cytomegalovirus glycoprotein B (gB) induced by adenovirus and vaccinia virus recombinants expressing gB

Robert F. Rando^2,

The¹ Wistar Institute of Anatomy and Biology, 3601 Spruce Street, Philadelphia, Pennsylvania 19104-4268, U.S.A.
² National Institute of Dental Research, 9000 Rockville Pike, Bethesda, Maryland 20892, U.S.A.
³ Institut Pasteur, Merieux, 1541 Avenue Marcel-Merieux, 69280 Marcy-l'Etoile, France
and⁴ Virogenetics Corporation, Rensselaer Technology Park, 465 Jordan Road, Troy, New York 12180, U.S.A.

A murine model of the cytotoxic T lymphocyte (CTL) response to glycoprotein B (gB) of human cytomegalovirus (HCMV) was developed based on the use of adenovirus (Ad) and vaccinia virus (Vac) recombinants expressing gB. Mice of different major histocompatibility haplotypes [CBA (H-2^k), BALB/k (H-2^k) and BALB/c (H-2^d)] infected with the Ad-gB recombinant developed an Ad-specific CTL response. However, only the H-2^k mice developed a significant HCMV gB-specific CTL response, as indicated by the major histocompatibility complex class I-restricted lysis of Vac strain Copenhagen (VacC)-gB recombinant-infected target cells by H-2^k mouse immune spleen cells. The VacC-gB recombinant elicited only a weak gB-specific CTL response in these mice, indicating that the observed gB-specific CTL response in mice is dependent on the expression vector used for immunization. The gB-specific cytotoxicity observed in H-2^k mice was mediated by the CD8 lymphocyte subset.

Present address: Triplex Pharmaceutical Corporation, 9391 Grogen's Mill Road, The Woodlands, Texas 77380, U.S.A.

Received 1 June 1993; accepted 20 July 1993.

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