| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Virology Swine Research Unit
and2 Virology Cattle Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, P.O. Box 70, 2300 Dayton Avenue, Ames, Iowa 50010, U.S.A.
Three isolates of feline calicivirus (FCV) designated NADC, KCD and CFI/68 were compared for biochemical, serological and genetic variation within the capsid protein gene. The Mr of the capsid protein from purified virions was approximately 66000 for the NADC virus isolate, which differed slightly from the relative mobilities of the purified capsid proteins of the KCD and CFI/68 isolates. Polyclonal antisera from either cats infected or rabbits hyperimmunized with the CFI/68 isolate cross-reacted with all three isolates by Western blot analysis. However, these polyclonal antisera to CFI/68 varied considerably in their virus-neutralization titres to the KCD and NADC isolates. Nucleotide sequence data confirmed the genetic variability among these FCV isolates. Comparison of the predicted amino acid sequence of the capsid protein among isolates revealed two regions of sequence divergence that probably contain the antigenically variable determinants. These hypervariable regions may vary by as much as 55% among isolates of FCV. The amino acid sequence diversity in the hypervariable regions of the KCD and NADC isolates correlated well with the virus-neutralization data and suggests that polyvalent vaccines may be more protective than the commonly used monovalent vaccines.
Present address: Southeast Poultry Research Laboratory, USDA, Agriculture Research Service, 934 College Station Road, P.O. Box 5657, Athens, Georgia 30604, U.S.A.
Received 12 March 1993;
accepted 9 July 1993.
This article has been cited by other articles:
|
|
 |
|
  D. Bhella, D. Gatherer, Y. Chaudhry, R. Pink, and I. G. Goodfellow Structural Insights into Calicivirus Attachment and Uncoating J. Virol., August 15, 2008; 82(16): 8051 - 8058. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. P. Coyne, D. Edwards, A. D. Radford, P. Cripps, D. Jones, J. L. N. Wood, R. M. Gaskell, and S. Dawson Longitudinal Molecular Epidemiological Analysis of Feline Calicivirus Infection in an Animal Shelter: a Model for Investigating Calicivirus Transmission within High-Density, High-Turnover Populations J. Clin. Microbiol., October 1, 2007; 45(10): 3239 - 3244. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. P. Coyne, R. M. Gaskell, S. Dawson, C. J. Porter, and A. D. Radford Evolutionary Mechanisms of Persistence and Diversification of a Calicivirus within Endemically Infected Natural Host Populations J. Virol., February 15, 2007; 81(4): 1961 - 1971. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Ossiboff, A. Sheh, J. Shotton, P. A. Pesavento, and J. S. L. Parker Feline caliciviruses (FCVs) isolated from cats with virulent systemic disease possess in vitro phenotypes distinct from those of other FCV isolates J. Gen. Virol., February 1, 2007; 88(2): 506 - 517. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. P. Coyne, F. C. Reed, C. J. Porter, S. Dawson, R. M. Gaskell, and A. D. Radford Recombination of Feline calicivirus within an endemically infected cat colony. J. Gen. Virol., April 1, 2006; 87(Pt 4): 921 - 926. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Schwantes, U. Truyen, J. Weikel, C. Weiss, and M. Lochelt Application of Chimeric Feline Foamy Virus-Based Retroviral Vectors for the Induction of Antiviral Immunity in Cats J. Virol., July 15, 2003; 77(14): 7830 - 7842. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Matsuura, Y. Tohya, M. Mochizuki, K. Takase, and T. Sugimura Identification of conformational neutralizing epitopes on the capsid protein of canine calicivirus J. Gen. Virol., July 1, 2001; 82(7): 1695 - 1702. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Neill, S. V. Sosnovtsev, and K. Y. Green Recovery and Altered Neutralization Specificities of Chimeric Viruses Containing Capsid Protein Domain Exchanges from Antigenically Distinct Strains of Feline Calicivirus J. Virol., February 1, 2000; 74(3): 1079 - 1084. [Abstract] [Full Text]
|
|
|
|
|
|
A. D. Radford, K. Willoughby, S. Dawson, C. McCracken, and R. M. Gaskell The Capsid Gene of Feline Calicivirus Contains Linear B-Cell Epitopes in both Variable and Conserved Regions J. Virol., October 1, 1999; 73(10): 8496 - 8502. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Geissler, K. Schneider, A. Fleuchaus, C. R. Parrish, G. Sutter, and U. Truyen Feline Calicivirus Capsid Protein Expression and Capsid Assembly in Cultured Feline Cells J. Virol., January 1, 1999; 73(1): 834 - 838. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
