HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Franco, M. A. Articles by Cohen, J. Search for Related Content PubMed PubMed Citation Articles by Franco, M. A. Articles by Cohen, J. Agricola Articles by Franco, M. A. Articles by Cohen, J.

An immunodominant cytotoxic T cell epitope on the VP7 rotavirus protein overlaps the H2 signal peptide

¹ Laboratoire de Virologie et Immunologie Moléculaires INRA, C.R.J., Domaine de Vilvert, 78350 Jouy-en-Josas, France
and² Departamento de Medicina Interna, Universidad de Navarra, Pamplona, Spain

C57BL/6 (H-2^b) mice were primed with the bovine RF strain of rotavirus to study the induction of CD8⁺ cytotoxic T lymphocytes (CTLs). These rotavirusspecific CTLs were detected only after in vitro restimulation with the virus. Using a recombinant vaccinia virus we identified the RF VP7 protein as a major target of these CTLs. The response against this protein was obtained also after in vitro restimulation with simian SA11 and human WA strains of rotavirus. Using published D^b and K^b allele-specific motifs to predict possible CTL epitopes in the RF VP7 protein, we synthesized and tested 18 predicted peptides of VP7. Only one peptide was able to sensitize target cells at a concentration below 5 x 10^-7 M. This CTL epitope was also induced by immunization with the RF VP7 expressed with a baculovirus vector, and was shown to be immunodominant by its capacity to inhibit, in an unlabelled target assay, the bulk response against cells infected with recombinant vaccinia virus expressing VP7. This CTL epitope overlaps the H2 signal peptide of the protein.

Received 4 May 1993; accepted 28 July 1993.

This article has been cited by other articles:

				S. Libersou, X. Siebert, M. Ouldali, L. F. Estrozi, J. Navaza, A. Charpilienne, P. Garnier, D. Poncet, and J. Lepault Geometric Mismatches within the Concentric Layers of Rotavirus Particles: a Potential Regulatory Switch of Viral Particle Transcription Activity J. Virol., March 15, 2008; 82(6): 2844 - 2852. [Abstract] [Full Text] [PDF]

				M. C. Jaimes, N. Feng, and H. B. Greenberg Characterization of Homologous and Heterologous Rotavirus-Specific T-Cell Responses in Infant and Adult Mice J. Virol., April 15, 2005; 79(8): 4568 - 4579. [Abstract] [Full Text] [PDF]

				B. S. Coulson, P. D. Witterick, Y. Tan, M. J. Hewish, J. N. Mountford, L. C. Harrison, and M. C. Honeyman Growth of Rotaviruses in Primary Pancreatic Cells J. Virol., August 12, 2002; 76(18): 9537 - 9544. [Abstract] [Full Text] [PDF]

				A. Charpilienne, M. Nejmeddine, M. Berois, N. Parez, E. Neumann, E. Hewat, G. Trugnan, and J. Cohen Individual Rotavirus-like Particles Containing 120 Molecules of Fluorescent Protein Are Visible in Living Cells J. Biol. Chem., July 27, 2001; 276(31): 29361 - 29367. [Abstract] [Full Text] [PDF]