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J Gen Virol 74 (1993), 2725-2729; DOI 10.1099/0022-1317-74-12-2725
© 1993 Society for General Microbiology
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Replication of the DNA A component of African cassava mosaic virus in a heterologous system

Uta Paszkowski{dagger}, Shi-Bo Zhang{ddagger}, Ingo Potrykus and Jerzy Paszkowski{dagger}

Institute for Plant Sciences, Swiss Federal Institute of Technology, ETH-Zurich, Universitätsstrasse 2, 8092 Zurich, Switzerland

The capacity for autonomous replication of the DNA A of African cassava mosaic virus (ACMV), a member of the bipartite geminiviruses infecting dicotyledonous plants, has been compared in host and non-host cells. A derivative of the ACMV DNA A was transfected into tobacco and maize protoplasts. Although ACMV is not able to infect maize, replication of the DNA A in maize protoplasts was observed to occur. The efficiency of replication was 10 to 20% of that seen in tobacco protoplasts. In both plant systems, replication was detected after the onset of cell division. ACMV replication in maize cells was compared to that of wheat dwarf virus and found to be 10 to 20% of that observed with the monocotyledon-specific virus. Insertion of 1165 bp of non-viral DNA into the ACMV DNA A prevented replication in maize but not in tobacco.

{dagger} Present address: Friedrich Miescher Institute, P.O. Box 2543, 4002 Basel, Switzerland.

{ddagger} Present address: 202 Pesticide Research Center, Department of Crop and Soil Sciences, Michigan State University, East Lansing, Michigan 48824, U.S.A.

Received 1 April 1993; accepted 20 July 1993.




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Copyright © 1993 by the Society for General Microbiology.