| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Plant Pathology
and2 Department of Biological Sciences, University of Kentucky, Lexington, Kentucky 40546, U.S.A.
The N-terminal portion (P1-HC-Pro-P3) of the tobacco vein mottling virus (TVMV) polyprotein was expressed in insect cells and larvae by a recombinant baculovirus. The proteases necessary to process this TVMV polyprotein fragment were active in insect cells, since mature P1, HC-Pro and P3 proteins were detected by specific antisera in Western blots. Antisera to P1, HC-Pro and P3 also recognized polypeptides with apparent Mr values predicted for the intermediate processing products of the polyprotein fragment. The results of this study indicate that the autocatalytic processing of TVMV HC-Pro from the polyprotein is supported by insect cells. Helper component activity in extracts of cells infected with recombinant baculovirus was not detected by aphid transmission assay.
Present address: DLO Research Institute for Plant Protection, P.O. Box 9060, 6700 GW Wageningen, The Netherlands.
Received 7 June 1993;
accepted 4 August 1993.
This article has been cited by other articles:
|
|
 |
|
  E. Goytia, L. Fernandez-Calvino, B. Martinez-Garcia, D. Lopez-Abella, and J. J. Lopez-Moya Production of plum pox virus HC-Pro functionally active for aphid transmission in a transient-expression system. J. Gen. Virol., November 1, 2006; 87(Pt 11): 3413 - 3423. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Ruiz-Ferrer, J. Boskovic, C. Alfonso, G. Rivas, O. Llorca, D. Lopez-Abella, and J. J. Lopez-Moya Structural Analysis of Tobacco Etch Potyvirus HC-Pro Oligomers Involved in Aphid Transmission J. Virol., March 15, 2005; 79(6): 3758 - 3765. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Ruiz-Ferrer, E. Goytia, B. Martinez-Garcia, D. Lopez-Abella, and J. J. Lopez-Moya Expression of functionally active helper component protein of Tobacco etch potyvirus in the yeast Pichia pastoris J. Gen. Virol., January 1, 2004; 85(1): 241 - 249. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Merits, M.-L. Rajamaki, P. Lindholm, P. Runeberg-Roos, T. Kekarainen, P. Puustinen, K. Makelainen, J. P. T. Valkonen, and M. Saarma Proteolytic processing of potyviral proteins and polyprotein processing intermediates in insect and plant cells J. Gen. Virol., May 1, 2002; 83(5): 1211 - 1221. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Sasaya, L. Torrance, G. Cowan, and A. Ziegler Aphid transmission studies using helper component proteins of Potato virus Y expressed from a vector derived from Potato virus X J. Gen. Virol., April 1, 2000; 81(4): 1115 - 1119. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
