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J Gen Virol 74 (1993), 201-210; DOI 10.1099/0022-1317-74-2-201
© 1993 Society for General Microbiology
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Characterization of a New York ovine lentivirus isolate

B. J. Campbell1,{dagger}, D. R. Thompson2, J. R. Williams1, S. G. Campbell1 and R. J. Avery1

1 Department of Microbiology, Immunology and Parasitology, College of Veterinary Medicine, 615 VRT, Cornell University, Ithaca, New York 14853, U.S.A.
and2 Merck, Sharp & Dohme Veterinary Research and Development Laboratory, PO Box 135, Ingleburn, New South Wales, Australia 2565

A lentivirus has been isolated from a Finnish ewe with ovine progressive pneumonia in a closed upstate New York flock. We demonstrated that the virus, designated ovine lentivirus strain CU1 (OLV-CU1), is biologically, biochemically and molecularly related to, but distinct from, previously described sheep and goat lentiviruses. Nine of 32 ewes (from the affected flock) with precipitating antibodies for ovine lentivirus also produced antibodies that were able to neutralize the infectivity of OLV-CU1. The virus replicated in cultured sheep fibroblasts and caused the formation of large multi-nucleated cells. OLV-CU1-specific RNA transcripts found in infected cells and virion antigenic proteins were similar to those of other small ruminant lentiviruses. However, the virus was distinguished from other isolates at the DNA level by nucleic acid hybridization, restriction endonuclease mapping and partial sequencing of the virus genome.

{dagger} Present address: LID/NAIAID/NIH Twinbrook II, 12441 Parklawn Drive, Rockville, Maryland 20852, U.S.A.

Received 20 May 1992; accepted 5 October 1992.




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Copyright © 1993 by the Society for General Microbiology.