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Molecular cloning and sequence analysis of a segment from Andean potato mottle virus B RNA encoding the putative RNA polymerase

¹ Departamento de Genética, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Brazil
² Departamento de Genética, FIOCRUZ, Universidade Federal Rural do Rio de Janeiro, Brazil
³ Departamento de Fitopatologia, Universidade Federal Rural do Rio de Janeiro, Brazil
⁴ Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Brazil
and⁵ Laboratoirum voor Genetica, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium

Andean potato mottle virus (APMV), an endemic South American comovirus, has a bipartite genome consisting of two plus-strand RNA molecules (M and B RNA). We have cloned the 3' half of the B RNA and identified the complete sequence of the putative APMV RNA polymerase. The RNA polymerase gene is part of a large polyprotein-encoding open reading frame. The putative, mature RNA polymerase, as deduced by comparison with the related cowpea mosaic virus (CPMV), type member of the comovirus group, is 703 amino acids long and shows a large degree of similarity with CPMV and other RNA polymerases.

Received 8 June 1992; accepted 29 September 1992.