HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Zhang, C. X. Articles by Morgan, A. J. Search for Related Content PubMed PubMed Citation Articles by Zhang, C. X. Articles by Morgan, A. J. Agricola Articles by Zhang, C. X. Articles by Morgan, A. J.

Analysis of Epstein—Barr virus gene transcription in lymphoma induced by the virus in the cottontop tamarin by construction of a cDNA library with RNA extracted from a tumour biopsy

Chang X. Zhang

Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, U.K.

Inoculation of the cottontop tamarin with Epstein—Barr virus (EBV) gives rise to the development of mono- and/or oligoclonal large cell malignant lymphoma. A cDNA library was generated with the RNA extracted from an EBV-induced tamarin lymphoma biopsy in order to study the transcripts expressed in the tumour tissue. Fifteen EBV-specific cDNA clones were localized in the corresponding viral genomic fragments. Among them, two correspond to the EBNA-2 gene, and two others to the latent membrane protein gene. The majority of the cDNA clones were localized in the BamHI A fragment which has not been associated with latent expression. Furthermore, cDNAs were also found from the BamHI D and I fragments. Sequence analysis of the cDNAs localized in BamHI A showed that they correspond to a rightward transcript in the BALF-3 region, with the one clone that was sequenced containing four exons and three introns. The above results were confirmed by testing three different biopsies with the rapid amplification of cDNA ends-PCR method.

Present address: Laboratoire de Virologie Moléculaire, UMR 30, CNRS-UCLB, Faculté de Médecine Alexis Carrel, Rue G. Paradin, 69372, Lyon, Cedex 08, France.

Received 13 August 1992; accepted 26 October 1992.

This article has been cited by other articles:

				G. Delhon, M. P. Moraes, Z. Lu, C. L. Afonso, E. F. Flores, R. Weiblen, G. F. Kutish, and D. L. Rock Genome of Bovine Herpesvirus 5 J. Virol., October 1, 2003; 77(19): 10339 - 10347. [Abstract] [Full Text] [PDF]

				O. de Jesus, P. R. Smith, L. C. Spender, C. Elgueta Karstegl, H. H. Niller, D. Huang, and P. J. Farrell Updated Epstein-Barr virus (EBV) DNA sequence and analysis of a promoter for the BART (CST, BARF0) RNAs of EBV J. Gen. Virol., June 1, 2003; 84(6): 1443 - 1450. [Abstract] [Full Text] [PDF]

				P. R. Smith, O. de Jesus, D. Turner, M. Hollyoake, C. E. Karstegl, B. E. Griffin, L. Karran, Y. Wang, S. D. Hayward, and P. J. Farrell Structure and Coding Content of CST (BART) Family RNAs of Epstein-Barr Virus J. Virol., April 1, 2000; 74(7): 3082 - 3092. [Abstract] [Full Text]

				Q. Tao, K. D. Robertson, A. Manns, A. Hildesheim, and R. F. Ambinder Epstein-Barr Virus (EBV) in Endemic Burkitt's Lymphoma: Molecular Analysis of Primary Tumor Tissue Blood, February 15, 1998; 91(4): 1373 - 1381. [Abstract] [Full Text] [PDF]