HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wolstenholme, A. J. Articles by Douglass, W. Search for Related Content PubMed PubMed Citation Articles by Wolstenholme, A. J. Articles by Douglass, W. Agricola Articles by Wolstenholme, A. J. Articles by Douglass, W.

Expression of hepatitis A virus poly(U) polymerase in the periplasmic space of Escherichia coli

Department of Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, U.K.

A cDNA containing almost all of the hepatitis A virus (HAV) P3 sequences was expressed as a fusion protein with Protein A. A novel poly(U) polymerase activity was detected in the periplasmic space of Escherichia coli cells transformed with this plasmid, and this activity showed many of the expected properties of a picornavirus 3D^pol. A number of HAV-specific polypeptides were detected in these cells, and it is unclear which of these was responsible for the polymerase activity.

Received 5 May 1992; accepted 19 October 1992.