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J Gen Virol 74 (1993), 549-554; DOI 10.1099/0022-1317-74-4-549
© 1993 Society for General Microbiology
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Maize stripe virus RNA5 is of negative polarity and encodes a highly basic protein

Layne Huiet1,{dagger}, James H. Tsai2 and Bryce W. Falk1

1 Department of Plant Pathology, University of California, Davis, California 95616
and2 University of Florida, Fort Lauderdale Research and Education Center, Fort Lauderdale, Florida 33314, U.S.A.

The entire 1317 nucleotide sequence of the maize stripe virus (MStV) RNA5 was determined. Only one open reading frame (ORF) was identified and was found in the viral complementary RNA (vcRNA). This ORF appears to encode a protein of Mr 44237, hereafter referred to as NS5. In vitro translation of transcripts representing nearly full-length RNA5 vcRNA yielded products of the predicted size, as well as some smaller, less prominent products. No products were identified from transcripts of viral polarity. RNA hybridization analyses of MStV-infected Zea mays revealed RNAs corresponding only to full-length RNA5, but both positive and negative polarity RNAs were abundant. Analysis of the NS5 amino acid sequence revealed that it is extremely basic, containing 21% arginine and lysine. Database comparisons showed that NS5 had no significant similarity to other protein sequences.

{dagger} Present address: Centre for Engineering Plants for Resistance Against Pathogens (CEPRAP), 1930 5th Street, University of California, Davis, California 95616, U.S.A.

Received 9 October 1992; accepted 8 December 1992.




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Copyright © 1993 by the Society for General Microbiology.