HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Leppard, K. N. Search for Related Content PubMed PubMed Citation Articles by Leppard, K. N. Agricola Articles by Leppard, K. N.

Selective effects on adenovirus late gene expression of deleting the E1b 55K protein

Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, U.K.

The E1b gene of human adenovirus 5 (Ad5) encodes a 55K product previously shown to be required for the efficient accumulation of mRNAs derived from the major late primary transcript in the cytoplasm of infected cells. This finding is extended here to include the transcripts from other viral promoters activated during the late phase of infection. Conversely, accumulation of mRNA derived from the major late promoter at early times is not dependent on this E1b function. Cytoplasmic levels of the various differentially spliced products of the major late unit are not equally dependent on the E1b 55K protein. Rather, the longest mRNA species within each 3'-coterminal family shows the greatest dependence. These findings support a model in which the Ad5 E1b 55K protein acts to facilitate the movement of mature viral mRNA away from the nuclear matrix of the infected cell.

Received 16 November 1992; accepted 24 November 1992.

This article has been cited by other articles:

				R. A. Gonzalez and S. J. Flint Effects of Mutations in the Adenoviral E1B 55-Kilodalton Protein Coding Sequence on Viral Late mRNA Metabolism J. Virol., March 27, 2002; 76(9): 4507 - 4519. [Abstract] [Full Text] [PDF]

				Y. Shen, G. Kitzes, J. A. Nye, A. Fattaey, and T. Hermiston Analyses of Single-Amino-Acid Substitution Mutants of Adenovirus Type 5 E1B-55K Protein J. Virol., May 1, 2001; 75(9): 4297 - 4307. [Abstract] [Full Text]

				M. Brunori, M. Malerba, H. Kashiwazaki, and R. Iggo Replicating Adenoviruses That Target Tumors with Constitutive Activation of the wnt Signaling Pathway J. Virol., March 15, 2001; 75(6): 2857 - 2865. [Abstract] [Full Text]

				K. Leppard and R. Everett The adenovirus type 5 E1b 55K and E4 Orf3 proteins associate in infected cells and affect ND10 components J. Gen. Virol., April 1, 1999; 80(4): 997 - 1008. [Abstract]

				J. J. Horridge and K. N. Leppard RNA-Binding Activity of the E1B 55-Kilodalton Protein from Human Adenovirus Type 5 J. Virol., November 1, 1998; 72(11): 9374 - 9379. [Abstract] [Full Text] [PDF]

				S. Gabler, H. Schutt, P. Groitl, H. Wolf, T. Shenk, and T. Dobner E1B 55-Kilodalton-Associated Protein: a Cellular Protein with RNA-Binding Activity Implicated in Nucleocytoplasmic Transport of Adenovirus and Cellular mRNAs J. Virol., October 1, 1998; 72(10): 7960 - 7971. [Abstract] [Full Text] [PDF]

				W. Huang and S. J. Flint The Tripartite Leader Sequence of Subgroup C Adenovirus Major Late mRNAs Can Increase the Efficiency of mRNA Export J. Virol., January 1, 1998; 72(1): 225 - 235. [Abstract] [Full Text] [PDF]