| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
2 protein of reovirus
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Building 38A, 8600 Rockville Pike, Bethesda, Maryland 20894, U.S.A.
A sequence motif that is conserved in a number of S-adenosylmethionine (SAM)-utilizing methyltransferases and is implicated in SAM binding was identified in the N-terminal portion of NS5 proteins of flaviviruses and in 
2 protein of reovirus. An additional conserved motif was shared by these viral proteins and two distinct groups of methyltransferases including as the prototypes Rhodobacter capsulatus hydroxyneurosporene methylase (crtF gene product) and yeast 3,4-dihydroxy-5-hexaprenylbenzoate methylase (COQ3 gene product), respectively. Statistically significant similarity was revealed between the region of flavivirus NS5 containing the SAM-binding motif and a newly characterized family of putative methyltransferases from bacteria, yeast and plants, which is related to the Coq3 group. Amino acid sequence signatures were derived that are unique for NS5 proteins and different subsets of (putative) cellular methyltransferases. It is hypothesized that the N-terminal domain of NS5 is a methyltransferase involved in viral RNA capping. Thus NS5 may be a two-domain protein, with its C-terminal domain comprising the RNA-dependent RNA polymerase. The putative methyltransferase domain of flaviviruses is unrelated to the methyltransferase domain previously characterized in positive-strand RNA viruses of the alphavirus-like supergroup. The lack of sequence similarity and different location of the putative methyltransferase domain underscores the drastic difference in the genome layout of flaviviruses and alphaviruses. The identification of the putative methyltransferase domain in reovirus 2 protein is compatible with the available evidence that this protein is the viral capping enzyme.
Permanent address: Institute of Microbiology, Russian Academy of Sciences, Moscow, Russia.
Received 7 September 1992;
accepted 27 November 1992.
This article has been cited by other articles:
|
|
 |
|
  H. Kroschewski, S. P. Lim, R. E. Butcher, T. L. Yap, J. Lescar, P. J. Wright, S. G. Vasudevan, and A. D. Davidson Mutagenesis of the Dengue Virus Type 2 NS5 Methyltransferase Domain J. Biol. Chem., July 11, 2008; 283(28): 19410 - 19421. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Mastrangelo, M. Bollati, M. Milani, B. Selisko, F. Peyrane, B. Canard, G. Grard, X. de Lamballerie, and M. Bolognesi Structural bases for substrate recognition and activity in Meaban virus nucleoside-2'-O-methyltransferase Protein Sci., June 1, 2007; 16(6): 1133 - 1145. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Emara and M. A. Brinton Interaction of TIA-1/TIAR with West Nile and dengue virus products in infected cells interferes with stress granule formation and processing body assembly PNAS, May 22, 2007; 104(21): 9041 - 9046. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. E. Murray and M. L. Nibert Guanidine Hydrochloride Inhibits Mammalian Orthoreovirus Growth by Reversibly Blocking the Synthesis of Double-Stranded RNA J. Virol., May 1, 2007; 81(9): 4572 - 4584. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Miller, S. Kastner, J. Krijnse-Locker, S. Buhler, and R. Bartenschlager The Non-structural Protein 4A of Dengue Virus Is an Integral Membrane Protein Inducing Membrane Alterations in a 2K-regulated Manner J. Biol. Chem., March 23, 2007; 282(12): 8873 - 8882. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. P. Pijlman, N. Kondratieva, and A. A. Khromykh Translation of the Flavivirus Kunjin NS3 Gene in cis but Not Its RNA Sequence or Secondary Structure Is Essential for Efficient RNA Packaging J. Virol., November 15, 2006; 80(22): 11255 - 11264. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R.-J. Lin, B.-L. Chang, H.-P. Yu, C.-L. Liao, and Y.-L. Lin Blocking of Interferon-Induced Jak-Stat Signaling by Japanese Encephalitis Virus NS5 through a Protein Tyrosine Phosphatase-Mediated Mechanism. J. Virol., June 1, 2006; 80(12): 5908 - 5918. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Puig-Basagoiti, M. Tilgner, B. M. Forshey, S. M. Philpott, N. G. Espina, D. E. Wentworth, S. J. Goebel, P. S. Masters, B. Falgout, P. Ren, et al. Triaryl pyrazoline compound inhibits flavivirus RNA replication. Antimicrob. Agents Chemother., April 1, 2006; 50(4): 1320 - 1329. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Rumyantsev, B. R. Murphy, and A. G. Pletnev A Tick-Borne Langat Virus Mutant That Is Temperature Sensitive and Host Range Restricted in Neuroblastoma Cells and Lacks Neuroinvasiveness for Immunodeficient Mice J. Virol., February 1, 2006; 80(3): 1427 - 1439. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Medin, K. A. Fitzgerald, and A. L. Rothman Dengue Virus Nonstructural Protein NS5 Induces Interleukin-8 Transcription and Secretion J. Virol., September 1, 2005; 79(17): 11053 - 11061. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Alcon-LePoder, M.-T. Drouet, P. Roux, M.-P. Frenkiel, M. Arborio, A.-M. Durand-Schneider, M. Maurice, I. Le Blanc, J. Gruenberg, and M. Flamand The Secreted Form of Dengue Virus Nonstructural Protein NS1 Is Endocytosed by Hepatocytes and Accumulates in Late Endosomes: Implications for Viral Infectivity J. Virol., September 1, 2005; 79(17): 11403 - 11411. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tilgner and P.-Y. Shi Structure and Function of the 3' Terminal Six Nucleotides of the West Nile Virus Genome in Viral Replication J. Virol., August 1, 2004; 78(15): 8159 - 8171. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nomaguchi, T. Teramoto, L. Yu, L. Markoff, and R. Padmanabhan Requirements for West Nile Virus (-)- and (+)-Strand Subgenomic RNA Synthesis in Vitro by the Viral RNA-dependent RNA Polymerase Expressed in Escherichia coli J. Biol. Chem., March 26, 2004; 279(13): 12141 - 12151. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K. Lo, M. Tilgner, and P.-Y. Shi Potential High-Throughput Assay for Screening Inhibitors of West Nile Virus Replication J. Virol., December 1, 2003; 77(23): 12901 - 12906. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nomaguchi, M. Ackermann, C. Yon, S. You, and R. Padmanbhan De Novo Synthesis of Negative-Strand RNA by Dengue Virus RNA-Dependent RNA Polymerase In Vitro: Nucleotide, Primer, and Template Parameters J. Virol., August 15, 2003; 77(16): 8831 - 8842. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kim, X. Zhang, V. E. Centonze, V. D. Bowman, S. Noble, T. S. Baker, and M. L. Nibert The Hydrophilic Amino-Terminal Arm of Reovirus Core Shell Protein {lambda}1 Is Dispensable for Particle Assembly J. Virol., October 25, 2002; 76(23): 12211 - 12222. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. J. Liu, P. L. Sedlak, N. Kondratieva, and A. A. Khromykh Complementation Analysis of the Flavivirus Kunjin NS3 and NS5 Proteins Defines the Minimal Regions Essential for Formation of a Replication Complex and Shows a Requirement of NS3 in cis for Virus Assembly J. Virol., October 2, 2002; 76(21): 10766 - 10775. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. de Lamballerie, S. Crochu, F. Billoir, J. Neyts, P. de Micco, E. C. Holmes, and E. A. Gould Genome sequence analysis of Tamana bat virus and its relationship with the genus Flavivirus J. Gen. Virol., October 1, 2002; 83(10): 2443 - 2454. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Brooks, M. Johansson, A. V. John, Y. Xu, D. A. Jans, and S. G. Vasudevan The Interdomain Region of Dengue NS5 Protein That Binds to the Viral Helicase NS3 Contains Independently Functional Importin beta 1 and Importin alpha /beta -Recognized Nuclear Localization Signals J. Biol. Chem., September 20, 2002; 277(39): 36399 - 36407. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Charlier, P. Leyssen, C. W. A. Pleij, P. Lemey, F. Billoir, K. Van Laethem, A.-M. Vandamme, E. De Clercq, X. de Lamballerie, and J. Neyts Complete genome sequence of Montana Myotis leukoencephalitis virus, phylogenetic analysis and comparative study of the 3' untranslated region of flaviviruses with no known vector J. Gen. Virol., August 1, 2002; 83(8): 1875 - 1885. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Markoff, X. Pang, H.-s. Houng, B. Falgout, R. Olsen, E. Jones, and S. Polo Derivation and Characterization of a Dengue Type 1 Host Range-Restricted Mutant Virus That Is Attenuated and Highly Immunogenic in Monkeys J. Virol., March 7, 2002; 76(7): 3318 - 3328. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Bujnicki and L. Rychlewski In silico identification, structure prediction and phylogenetic analysis of the 2'-O-ribose (cap 1) methyltransferase domain in the large structural protein of ssRNA negative-strand viruses Protein Eng. Des. Sel., February 1, 2002; 15(2): 101 - 108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Hanley, J. J. Lee, J. E. Blaney Jr, B. R. Murphy, and S. S. Whitehead Paired Charge-to-Alanine Mutagenesis of Dengue Virus Type 4 NS5 Generates Mutants with Temperature-Sensitive, Host Range, and Mouse Attenuation Phenotypes J. Virol., January 15, 2002; 76(2): 525 - 531. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ackermann and R. Padmanabhan De Novo Synthesis of RNA by the Dengue Virus RNA-dependent RNA Polymerase Exhibits Temperature Dependence at the Initiation but Not Elongation Phase J. Biol. Chem., October 19, 2001; 276(43): 39926 - 39937. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. N. Johnson, K. L. Johnson, R. Dasgupta, T. Gratsch, and L. A. Ball Comparisons among the larger genome segments of six nodaviruses and their encoded RNA replicases J. Gen. Virol., August 1, 2001; 82(8): 1855 - 1866. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Johansson, A. J. Brooks, D. A. Jans, and S. G. Vasudevan A small region of the dengue virus-encoded RNA-dependent RNA polymerase, NS5, confers interaction with both the nuclear transport receptor importin-{{beta}} and the viral helicase, NS3 J. Gen. Virol., April 1, 2001; 82(4): 735 - 745. [Abstract] [Full Text]
|
|
|
|
|
|
A. A. Khromykh, P. L. Sedlak, and E. G. Westaway cis- and trans-Acting Elements in Flavivirus RNA Replication J. Virol., April 1, 2000; 74(7): 3253 - 3263. [Abstract] [Full Text]
|
|
|
|
 |
|
 V. K. Viswanathan, P. H. Edelstein, C. D. Pope, and N. P. Cianciotto The Legionella pneumophila iraAB Locus Is Required for Iron Assimilation, Intracellular Infection, and Virulence Infect. Immun., March 1, 2000; 68(3): 1069 - 1079. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Luongo, K. M. Reinisch, S. C. Harrison, and M. L. Nibert Identification of the Guanylyltransferase Region and Active Site in Reovirus mRNA Capping Protein lambda 2 J. Biol. Chem., January 28, 2000; 275(4): 2804 - 2810. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Leyssen, E. De Clercq, and J. Neyts Perspectives for the Treatment of Infections with Flaviviridae Clin. Microbiol. Rev., January 1, 2000; 13(1): 67 - 82. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Khromykh, P. L. Sedlak, and E. G. Westaway trans-Complementation Analysis of the Flavivirus Kunjin ns5 Gene Reveals an Essential Role for Translation of Its N-Terminal Half in RNA Replication J. Virol., November 1, 1999; 73(11): 9247 - 9255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Mackenzie, M. K. Jones, and E. G. Westaway Markers for trans-Golgi Membranes and the Intermediate Compartment Localize to Induced Membranes with Distinct Replication Functions in Flavivirus-Infected Cells J. Virol., November 1, 1999; 73(11): 9555 - 9567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. C. Leitmeyer, D. W. Vaughn, D. M. Watts, R. Salas, I. Villalobos, de Chacon, C. Ramos, and R. Rico-Hesse Dengue Virus Structural Differences That Correlate with Pathogenesis J. Virol., June 1, 1999; 73(6): 4738 - 4747. [Abstract] [Full Text]
|
|
|
|
|
|
C. L. Luongo, C. M. Contreras, D. L. Farsetta, and M. L. Nibert Binding Site for S-Adenosyl-L-methionine in a Central Region of Mammalian Reovirus lambda 2 Protein. EVIDENCE FOR ACTIVITIES IN mRNA CAP METHYLATION J. Biol. Chem., September 11, 1998; 273(37): 23773 - 23780. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Khromykh, M. T. Kenney, and E. G. Westaway trans-Complementation of Flavivirus RNA Polymerase Gene NS5 by Using Kunjin Virus Replicon-Expressing BHK Cells J. Virol., September 1, 1998; 72(9): 7270 - 7279. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Pillutla, Z. Yue, E. Maldonado, and A. J. Shatkin Recombinant Human mRNA Cap Methyltransferase Binds Capping Enzyme/RNA Polymerase IIo Complexes J. Biol. Chem., August 21, 1998; 273(34): 21443 - 21446. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Rodriguez-Cousino, A. Solorzano, T. Fujimura, and R. Esteban Yeast Positive-stranded Virus-like RNA Replicons. 20 S AND 23 S RNA TERMINAL NUCLEOTIDE SEQUENCES AND 3' END SECONDARY STRUCTURES RESEMBLE THOSE OF RNA COLIPHAGES J. Biol. Chem., August 7, 1998; 273(32): 20363 - 20371. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Reed, A. E. Gorbalenya, and C. M. Rice The NS5A/NS5 Proteins of Viruses from Three Genera of the Family Flaviviridae Are Phosphorylated by Associated Serine/Threonine Kinases J. Virol., July 1, 1998; 72(7): 6199 - 6206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Farsetta, K. Chandran, and M. L. Nibert Transcriptional Activities of Reovirus RNA Polymerase in Recoated Cores. INITIATION AND ELONGATION ARE REGULATED BY SEPARATE MECHANISMS J. Biol. Chem., December 8, 2000; 275(50): 39693 - 39701. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Rho, S. Choi, Y. R. Seong, W.-K. Cho, S. H. Kim, and D.-S. Im PRMT5, Which Forms Distinct Homo-oligomers, Is a Member of the Protein-arginine Methyltransferase Family J. Biol. Chem., March 30, 2001; 276(14): 11393 - 11401. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. You, B. Falgout, L. Markoff, and R. Padmanabhan In Vitro RNA Synthesis from Exogenous Dengue Viral RNA Templates Requires Long Range Interactions between 5'- and 3'-Terminal Regions That Influence RNA Structure J. Biol. Chem., May 4, 2001; 276(19): 15581 - 15591. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
