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J Gen Virol 74 (1993), 811-818; DOI 10.1099/0022-1317-74-5-811
© 1993 Society for General Microbiology
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Characterization of the Antibody Response to the Latent Infection Terminal Proteins of Epstein-Barr Virus in Patients with Nasopharyngeal Carcinoma

Barbara Frech1,{dagger}, Ursula Zimber-Strobl2, Timothy T. C. Yip3, W. H. Lau3 and Nikolaus Mueller-Lantzsch1

1 Abteilung Virologie, Institut für Medizinische Mikrobiologie und Hygiene, Haus 47, Universitätskliniken des Saarlandes, D-6650 Homburg/Saar
2 Institut für klinische Molekularbiologie und Tumorgenetik, Hämatologikum der GSF, D-8000 München 70, Germany
and3 Institute of Radiology and Oncology, Queen Elizabeth Hospital, Wylie Road, Kowloon, Hong Kong

Human sera were tested for antibodies against the Epstein-Barr virus (EBV) latent infection terminal proteins (TPs). Anti-TP IgG and IgA antibodies were detected by an indirect immunofluorescence assay of insect cells expressing a recombinant TP1. Out of 301 human sera of patients with EBV-related and EBV-unrelated disorders, only sera from patients with nasopharyngeal carcinoma (NPC) (32/83; 38%) showed anti-TP antibodies. Studies on serial sera from German and Hong Kong NPC patients revealed a decline of anti-TP antibodies during tumour therapy, and none of these antibodies were identified in patients with early tumour stages or in remission. Comparative studies of TP1-specific polyclonal rabbit antisera and human TP-positive sera showed clear differences in the TP epitopes recognized by each. Human antisera contained antibodies only to native epitopes in exons 2 to 7 of TP1 whereas rabbit antisera reacted only with epitopes located in the first exon and, additionally, exhibited EBV strain specificities.

{dagger} Present address: Institut für Veterinärvirologie, CH-3012 Bern, Switzerland.

Received 28 August 1992; accepted 15 December 1992.


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Copyright © 1993 by the Society for General Microbiology.