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J Gen Virol 74 (1993), 889-892; DOI 10.1099/0022-1317-74-5-889
© 1993 Society for General Microbiology
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Cauliflower Mosaic Virus 35S Promoter-Controlled DNA Copies of Cowpea Mosaic Virus RNAs are Infectious on Plants

Johannes T. Dessens and George P. Lomonossoff

Department of Virus Research, John Innes Institute, John Innes Centre, Colney Lane, Norwich NR4 7UH, U.K.

Clones have been constructed that contain full-length cDNA copies of cowpea mosaic virus RNA1 and RNA2, downstream of the cauliflower mosaic virus 35S promoter. The clones, when linearized downstream of the viral sequences, give rise to cowpea mosaic virus-like symptoms when inoculated onto cowpea plants. Viral RNA and virions can be detected in the inoculated plants, demonstrating that the clones are directly infectious.

Received 10 November 1992; accepted 11 December 1992.


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Copyright © 1993 by the Society for General Microbiology.