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Site-Directed Mutagenesis of a Potyvirus Coat Protein and its Assembly in Escherichia Coli

CSIRO, Division of Biomolecular Engineering, Parkville Laboratory, 343 Royal Parade, Parkville 3052, Victoria, Australia

Multiple copies of the Johnsongrass mosaic virus coat protein synthesized in Escherichia coli can readily assemble to form potyvirus-like particles. This E. coli expression system has been used to identify some of the key amino acid residues, within the core region of the coat protein, required for assembly. The two charged residues R¹⁹⁴ and D²³⁸ previously proposed theoretically to be involved as a pair in the construction of a salt bridge crucial for the assembly process were targeted for site-directed mutagenesis. The results from our experiments suggest that the two residues are required for the assembly process but are not necessarily involved as a pair in a common salt bridge.

Received 15 September 1992; accepted 10 December 1992.

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