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>MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, U.K.
Tobacco mosaic virus mutant Ni 2519 has a selected temperature-sensitive defect in the spreading of local lesions, and additional reported defects in virus assembly and in host range. Here we show that the temperature-sensitive local lesion-spreading defect (which maps in the assembly origin within the gene encoding protein p30) is probably independent of mutations in the pseudoassembly origin or of host range defects resulting from other mutations in the coat protein. One new host range mutant has been isolated.
Present address: EMBL, Heidelberg, Germany.
> Present address: Division of Virology, Department of Pathology at Addenbrooke's Hospital, University of Cambridge, Cambridge CB2 2QQ, U.K.
Received 25 August 1992;
accepted 26 November 1992.
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