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Horticulture Research International, Worthing Road, Littlehampton, West Sussex BN17 6LP, U.K.
Several primary cell lines that support the complete replication of Cydia pomonella granulosis virus have been established from one culture of C. pomonella embryonic cells. Virus passaged three times in cells and once in larvae showed no change in restriction enzyme fragment patterns. Stages in virus replication observed by electron microscopy resembled those from in vivo studies. Cell lines that were maintained at or below 21 °C retained susceptibility to virus over a period of 4 years whereas the same cell lines maintained at 27 °C gradually lost their susceptibility and eventually could not be infected at all.
Received 16 November 1992;
accepted 25 March 1993.
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