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1 Department of Microbiology, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3
and2 Research Station, Agriculture Canada, 6660 Northwest Marine Drive, Vancouver, British Columbia, Canada V6T 1X2
The presence of symptom-attenuating defective interfering (DI) RNAs in a laboratory culture of cucumber necrosis tombusvirus (CNV) was confirmed. Sequencing of cDNA clones of these DI RNAs revealed that CNV DI RNAs retained sequences from the CNV 5'-untranslated and 3'-terminal regions as well as a portion of the coding region for the 92K protein. Similar sequence arrangements were also observed in symptom-attenuating DI RNAs generated de novo from synthetic wild-type CNV transcripts. A comparison of the sequences and biological activities of these CNV DI RNAs is presented. In co-infections of synthetic wild-type CNV and CNV DI RNAs, prominent RNA species of a higher Mr than the DI RNA used in the co-infection were found. The possible nature of these RNA species is discussed.
Received 13 January 1992;
accepted 23 March 1993.
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