HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Tang, J. R. Articles by Trépo, C. Search for Related Content PubMed PubMed Citation Articles by Tang, J. R. Articles by Trépo, C. Agricola Articles by Tang, J. R. Articles by Trépo, C.

Discovery of a novel point mutation changing the HDAg expression of a hepatitis delta virus isolate from Central African Republic

Institut National de la Santé et de la Recherche Médicale (INSERM) U 271, 151 Cours Albert Thomas, 69424 Lyon, France

None of the mutations so far discovered in several hepatitis delta virus (HDV) isolates appears to determine important changes in HDV specific protein (HDAg) expression, except for a putative mutation at nucleotide 1012 converting an amber stop codon (TAG) to a codon for tryptophan (TGG). Here we present the characterization of an HDV obtained from the liver of a woodchuck inoculated with sera from fulminant HDV patients in Central African Republic (CAR). By restriction enzyme analysis and sequencing of HDAg-coding region cDNA clones, we found that this HDV isolate bears a novel mutation (T to A) at nucleotide 1013 which converts the amber stop codon (TAG) to a codon for lysine (AAG). Comparison of these nucleotide sequences with those available from American, Japanese, Taiwanese, French, Italian and Nauru isolates showed a variability of 1.7 to 21.5% and 1.9 to 28.7% at the nucleic acid and amino acid levels, respectively. The HDAg-encoding sequence of the CAR isolate is closely related to that of the Italian HDV isolate. The in vitro expression of this HDV isolate resulted in a unique HDAg species (28K) which was identical with that characterized in vivo.

Received 4 January 1993; accepted 29 April 1993.

This article has been cited by other articles:

				S. VIANA, R. PARANA, R. C. MOREIRA, A. P. COMPRI, and V. MACEDO HIGH PREVALENCE OF HEPATITIS B VIRUS AND HEPATITIS D VIRUS IN THE WESTERN BRAZILIAN AMAZON Am J Trop Med Hyg, October 1, 2005; 73(4): 808 - 814. [Abstract] [Full Text] [PDF]

				J.-J. Mu, D.-S. Chen, and P.-J. Chen The Conserved Serine 177 in the Delta Antigen of Hepatitis Delta Virus Is One Putative Phosphorylation Site and Is Required for Efficient Viral RNA Replication J. Virol., October 1, 2001; 75(19): 9087 - 9095. [Abstract] [Full Text] [PDF]

				J.-J. Mu, H.-L. Wu, B.-L. Chiang, R.-P. Chang, D.-S. Chen, and P.-J. Chen Characterization of the Phosphorylated Forms and the Phosphorylated Residues of Hepatitis Delta Virus Delta Antigens J. Virol., December 1, 1999; 73(12): 10540 - 10545. [Abstract] [Full Text]