J Gen Virol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 74 (1993), 1959-1964; DOI 10.1099/0022-1317-74-9-1959
© 1993 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cullinane, A. A.
Right arrow Articles by Allen, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cullinane, A. A.
Right arrow Articles by Allen, G.
Agricola
Right arrow Articles by Cullinane, A. A.
Right arrow Articles by Allen, G.

The DNA sequence of the equine herpesvirus 4 gene encoding glycoprotein gp17/18, the homologue of herpes simplex virus glycoprotein gD

A. A. Cullinane1, J. Neilan1,{dagger}, L. Wilson1,{ddagger}, A. J. Davison2 and G. Allen3

1 Virology Unit, Irish Equine Centre, Johnstown, Naas, County Kildare, Ireland
and2 MRC Virology Unit, Institute of Virology, University of Glasgow, Church Street, Glasgow G11 5JR, U.K.
and3 108 Gluck Equine Research Center, Lexington, Kentucky, U.S.A.

The nucleotide sequence of the gene to the left of the gI gene of equine herpesvirus 4 (EHV-4) was determined. The gene encodes a peptide of 402 amino acids with an unprocessed Mr of 45323. The predicted polypeptide has several features of a glycoprotein including a hydrophobic signal sequence, a membrane spanning domain and four potential N-linked glycosylation sites within the proposed external domain. The predicted amino acid sequence of EHV-4 gD shows 83% identity with that of equine herpesvirus 1 gD. Conservation of the tertiary structure is suggested by the alignment of six cysteine residues with those of the gD of six other alphaherpesviruses. Screening a {lambda}gt11/EHV-4 expression library with monoclonal antibodies against several of the most abundant EHV-4 glycoproteins unequivocally identified the protein encoded by the EHV-4 gD gene as gp17/18.

{dagger} Present address: Plum Island Disease Center, P.O. Box 848, Greenport, New York 11944, U.S.A.

{ddagger} Present address: AFRC Institute for Animal Health, Compton, Newbury, Berkshire RG16 0NN, U.K.

Received 6 January 1993; accepted 11 May 1993.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1993 by the Society for General Microbiology.