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Electron microscopic evidence for budding process-independent assembly of double-shelled rotavirus particles during passage through endoplasmic reticulum membranes

¹ Clinical Research Division, Sendai National Hospital, Sendai 983
and² Department of Pediatrics, Institute for Tuberculosis and Cancer, Tohoku University, Sendai 983, Japan

Slowing down of the maturation process of human rotavirus particles on ice allowed the clear demonstration of two different assembly pathways through the endoplasmic reticulum (ER) membrane. One was the ‘enveloped’ and single-shelled (ss) particle assembly pathway, in which a transient envelope is acquired through the budding of subviral particles from the cytoplasm to the ER lumen, and later these ‘enveloped’ particles are released as ss particles in the ER lumen. The other was a double-shelled particle assembly pathway by which subviral particles acquire the outer capsid proteins during their transport across the ER membrane.

Received 28 January 1993; accepted 30 April 1993.