HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Marschall, M. Articles by Meier-Ewert, H. Search for Related Content PubMed PubMed Citation Articles by Marschall, M. Articles by Meier-Ewert, H. Agricola Articles by Marschall, M. Articles by Meier-Ewert, H.

Productive and non-productive phases during long-term persistence of influenza C virus

Abteilung für Virologie, Institut für Medizinische Mikrobiologie und Hygiene, Technische Universität München, Biedersteiner Straße 29, DW-8000 München 40, Germany

Persistent infection with a variant of influenza C/Ann Arbor/1/50 virus in MDCK cells has been previously reported. However, the precise molecular mechanism of persistence is still unknown. We show that the release of active progeny virus, as tested for by haemagglutination and acetylesterase profiles, does not take place in freshly seeded MDCK cells. Productive virus replication occurs simultaneously with massive production of structural proteins as shown by immunoprecipitation and immunofluorescence. PCR for the HEF structural protein-encoding segment 4 revealed that positive-sense RNA is present only during virus multiplication whereas negative-sense RNA appears to be constantly detectable. In this study we give initial evidence that influenza C virus can persist in the form of its genomic minus strand RNA, and plus strand transcription, protein synthesis and virus replication remain restricted to productive phases.

Received 16 February 1993; accepted 11 May 1993.

This article has been cited by other articles:

				M. S. Ibrahim, M. Watanabe, J. A. Palacios, W. Kamitani, S. Komoto, T. Kobayashi, K. Tomonaga, and K. Ikuta Varied Persistent Life Cycles of Borna Disease Virus in a Human Oligodendroglioma Cell Line J. Virol., March 19, 2002; 76(8): 3873 - 3880. [Abstract] [Full Text] [PDF]