HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Christensen, J. Articles by Uttenthal, A. Search for Related Content PubMed PubMed Citation Articles by Christensen, J. Articles by Uttenthal, A. Agricola Articles by Christensen, J. Articles by Uttenthal, A.

Production of mink enteritis parvovirus empty capsids by expression in a baculovirus vector system: a recombinant vaccine for mink enteritis parvovirus in mink

Åse Uttenthal^3,

¹ Laboratory of Virology and Immunology, Department of Veterinary Microbiology
² Laboratory of Molecular Pathobiology, Department of Pharmacology and Pathobiology, The Royal Veterinary and Agricultural University of Copenhagen, Bülowsvej 13, DK-1870 Frederiksberg C
and³ The Danish Fur Breeders Laboratory, Langagervej 74, DK-2600 Glostrup, Denmark

The VP-2 gene of mink enteritis parvovirus (MEV) was amplified by the polymerase chain reaction using MEV DNA isolated from the faeces of a naturally infected mink. Subsequently the VP-2 gene was cloned into a baculovirus expression vector. Recombinant baculo-viruses were isolated and the MEV VP-2 gene product was characterized after expression in Sf9 insect cells. The MEV VP-2 product had the same size as that reported for the wild-type MEV VP-2 protein and was recognized by convalescent sera from MEV-infected mink and a panel of monoclonal antibodies reactive to MEV. Furthermore, the VP-2 protein was able to form parvovirus-like particles, which had haemagglutinating properties comparable with the wild-type MEV. The cloned VP-2 gene was sequenced and only five nucleotide differences were found after alignment with the known sequences of the MEV type 1 and type 2 isolates. Surprisingly, the VP-2 gene encoded a valine and a tyrosine at amino acid positions 232 and 234, identical to the situation found in MEV type 1, but at position 300 there was a valine which is a determinant of MEV type 2. Immunization of mink with approximately 40000 haemagglutinating units of recombinant MEV VP-2 induced a measurable antibody response as tested by haemagglutination inhibition. Furthermore, the immunized mink did not excrete virus and did not develop clinical disease upon challenge with a virulent isolate of MEV.

Present address: National Veterinary Laboratory, Bülowsvej 27, DK-1790 Copenhagen V, Denmark.

Received 6 May 1993; accepted 30 August 1993.

This article has been cited by other articles:

				S. Hervas-Stubbs, P. Rueda, L. Lopez, and C. Leclerc Insect Baculoviruses Strongly Potentiate Adaptive Immune Responses by Inducing Type I IFN J. Immunol., February 15, 2007; 178(4): 2361 - 2369. [Abstract] [Full Text] [PDF]

				M. E. Bloom, S. M. Best, S. F. Hayes, R. D. Wells, J. B. Wolfinbarger, R. McKenna, and M. Agbandje-McKenna Identification of Aleutian Mink Disease Parvovirus Capsid Sequences Mediating Antibody-Dependent Enhancement of Infection, Virus Neutralization, and Immune Complex Formation J. Virol., November 15, 2001; 75(22): 11116 - 11127. [Abstract] [Full Text] [PDF]

				J. M. Fox and M. E. Bloom Identification of a Cell Surface Protein from Crandell Feline Kidney Cells That Specifically Binds Aleutian Mink Disease Parvovirus J. Virol., May 1, 1999; 73(5): 3835 - 3842. [Abstract] [Full Text]