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1 ICRF Papillomavirus Group, Department of Biology, University College London, London WC1E 6BT
2 ICRF Tumour Virus Group, Department of Pathology, University of Cambridge, Cambridge CB2 1QP, U.K.
3 Papillomavirus Research Unit, Lions Human Immunology Laboratories, University of Queensland, Princess Alexandra Hospital, Woolloongabba 4102, Australia
4 Ludwig Institute for Cancer Research, St Mary's Hospital Medical School, Norfolk Place, London W2 1PG
and5 ICRF Human Tumour Immunology Group, Courtauld Institute of Biochemistry, 91 Riding House Street, London W1P 8PT, U.K.
Immunization of mice with a recombinant vaccinia virus expressing the human papillomavirus type 16 (HPV-16) E6 gene elicits specific antibody, proliferative and cytotoxic T lymphocyte responses. T and B cell epitopes were mapped by using synthetic peptides. This study provides the background to future investigation aimed at developing prophylactic and therapeutic vaccines against HPV-16 infection and cervical cancer.
Present address: Department of Immunology, Windeyer Building, Cleveland Street, London WC1 6DB, U.K.
Received 10 April 1993;
accepted 30 August 1993.
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