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J Gen Virol 75 (1994), 165-169; DOI 10.1099/0022-1317-75-1-165
© 1994 Society for General Microbiology
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Delayed-type hypersensitivity response to human papillomavirus type 16 E6 protein in a mouse model

Mark A. Chambers1, Simon N. Stacey2, John R. Arrand2 and Margaret A. Stanley1

1 Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP
and2 Cancer Research Campaign Department of Molecular Biology, Paterson Institute for Cancer Research, Christie Hospital, Manchester M20 9BX, U.K.

A mouse model incorporating the epitheliotropic nature of human papillomavirus (HPV) infections has been used to study an immune response to HPV type 16 (HPV-16) E6 protein in vivo. Using a transplantation technique, a novel immortal keratinocyte cell line expressing the E6 protein has been grafted onto syngeneic mice to re-form a differentiated epithelium overlying a granulation tissue bed. By this approach the presentation of viral antigens to the immune system can be modelled in a way analogous to the natural infection. Here we report a delayed-type hypersensitivity (DTH) reaction in grafted mice challenged intradermally with a recombinant vaccinia virus expressing the HPV-16 E6 protein. The specificity of the response was confirmed by the absence of a DTH reaction to challenge with virus expressing either HPV-16 E7 or L1 protein.

Received 16 June 1993; accepted 30 August 1993.


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J. Hu, R. Han, N. M. Cladel, M. D. Pickel, and N. D. Christensen
Intracutaneous DNA Vaccination with the E8 Gene of Cottontail Rabbit Papillomavirus Induces Protective Immunity against Virus Challenge in Rabbits
J. Virol., June 5, 2002; 76(13): 6453 - 6459.
[Abstract] [Full Text] [PDF]


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