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Cloning of the Nilaparvata lugens reovirus genome: conserved terminal nucleotide sequences and nucleotide sequence of genome segment S10

¹ National Institute of Sericultural and Entomological Science
and² National Agriculture Research Center, Tsukuba, Ibaraki 305, Japan

The segmented double-stranded RNA genome of Nilaparvata lugens reovirus (NLRV) was cloned, and the nucleotide sequence of genome segment S10 and terminal nucleotide sequences of the rest of the segments were determined. Genome segment S10 of NLRV consisted of 1430 nucleotides with a single open reading frame extending for 1293 nucleotides from nucleotide 46. It encoded a polypeptide of 431 amino acids with an M_r of 49.4K, which was a non-structural protein. The plus-strand RNA of all genome segments had the same conserved trinucleotide 5' AGU- and hexanucleotide-GUUGUC 3' in the 5'- and 3'-terminal regions, respectively. These conserved terminal sequences resembled those found in the segments of the members of the genus Fijivirus.

Received 24 May 1993; accepted 1 September 1993.