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Characterization of proteins encoded by the short unique region of herpesvirus of turkeys byin vitroexpression

Vladimír Zelník

Jaromír Pastorek

Institute for Animal Health BBSRC, Compton Laboratory, Compton Nr. Newbury, Berkshire RG16 0NN, U.K.

Nine open reading frames mapping in the short unique (U_s) region of the genome of herpesvirus of turkeys (HVT) were expressed by in vitro transcription and translation. The observed M_rs of US10, SORF3 and US2 were as predicted from the sequence but there were discrepancies between the observed and predicted M_rs of US1, protein kinase, gI, gD and gE. These could be accounted for in most cases by post-translational and co-translational processing. Analysis of the synthesized products at different time points provided evidence for post-translational modification of HVT protein kinase. Translation in the presence of microsomal membranes resulted in co-translational processing of HVT gD, gI and gE by glycosylation and signal peptide cleavage.

Present address: Institute of Virology, Slovak Academy of Sciences, Dúbravská 9, Bratislava, 842 46, Slovak Republic.

Received 11 April 1994; accepted 16 May 1994.

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