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The nucleotide sequence of apple mosaic virus coat protein gene has no similarity with other Bromoviridae coat protein genes

¹ University of Illinois, Department of Horticulture, Plant and Animal Biotechnology, Urbana, Illinois 61801,
² University of Idaho, Department of Plant, Soil and Entomological Sciences, Moscow, Idaho 83843,
³ USDA/ARS/MWA, Crop Protection Research Unit, Turner Hall, Urbana, Illinois 61801
and⁴ University of Illinois, Department of Plant Pathology, Turner Hall, Urbana, Illinois 61801, U.S.A.

A double-stranded cDNA was synthesized from in vitro polyadenylated apple mosaic virus (ApMV) RNA 3 using oligo(dT) and sequence-specific primers, and was cloned into plasmid vectors. A set of overlapping cDNA clones was used to determine the nucleotide sequence of RNA 4. ApMV RNA 4 was found to contain an open reading frame (ORF) of 666 nucleotides, which was flanked by 5' and 3' non-translated sequences of 55 and 264 nucleotides, respectively. The ORF encoding the coat protein was identified by comparing the predicted amino acid sequence with that obtained from direct protein microsequencing of the native viral coat protein. The ORF encodes a protein with an M_r of 25056. The nucleotide sequence of the ApMV coat protein gene showed no similarity to those of alfalfa mosaic virus, tobacco streak virus (TSV), brome mosaic virus or cucumber mosaic virus. The predicted amino acid sequence of the amino-terminal region of the ApMV coat protein is basic, rich in cysteine residues and may contain a zinc finger motif similar to that found in TSV.

Received 27 January 1994; accepted 26 April 1994.

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