| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Institute of Virology, University of Glasgow, Church Street, Glasgow G11 5JR, U.K.
The Bunyamwera bunyavirus (BUN) M RNA genome segment encodes three proteins, two glycoproteins termed G1 and G2 and a non-structural protein called NSm, in the form of a polyprotein precursor that is cotranslationally cleaved to give the mature proteins. Indirect immunofluorescence experiments have shown that these proteins localize to the Golgi complex in BUN-infected cells. We have used a recombinant vaccinia virus (vTF7-3), which expresses bacteriophage T7 RNA polymerase, to drive the expression of plasmids containing either the entire BUN M segment cDNA or fragments that encode the G1, G2 and NSm proteins separately under control of the T7 promoter. After transfection of these plasmids into vTF7-3-infected cells, correctly sized and processed proteins were detected by immunoprecipitation with BUN-specific antibodies. Immunofluorescence experiments showed that G1, G2 and NSm localized to the Golgi when transiently expressed from the full-length cDNA. When G2 or NSm were expressed separately they also localized to the Golgi, but when G1 was expressed alone a staining pattern typical for the endoplasmic reticulum was obtained. However coexpression of G2 and G1 from independent plasmids resulted in G1 localizing to the Golgi. In contrast translocation of G1 to the Golgi was not observed when G1 was coexpressed with NSm, although NSm itself was still detected in the Golgi. Similar results were obtained when the proteins were expressed from transfected plasmids containing the G2-, NSm- or G1-coding sequences under control of the cytomegalovirus immediate-early promoter. The localization of G1 to the Golgi when coexpressed with G2 was confirmed by the loss of endoglycosidase H (endo H) sensitivity of G1 after approximately 60 min in a pulse-chase experiment; G1 remained sensitive to endo H when expressed either alone or in combination with NSm. These results suggest that G2 contains the Golgi targeting and/or retention signals and that G1 has to interact with this protein to localize to this cellular compartment.
Present address: Virus Research Centre, Kenya Medical Research Institute, P.O. Box 54628, Nairobi, Kenya.
Present address: Department of Molecular and Life Sciences, University of Abertay Dundee, Bell Street, Dundee DD1 1HG, U.K.
Received 22 June 1994;
accepted 19 August 1994.
This article has been cited by other articles:
|
|
 |
|
  A. K. Earley, W. M. Chan, and B. M. Ward The Vaccinia Virus B5 Protein Requires A34 for Efficient Intracellular Trafficking from the Endoplasmic Reticulum to the Site of Wrapping and Incorporation into Progeny Virions J. Virol., March 1, 2008; 82(5): 2161 - 2169. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Shi, A. Kohl, P. Li, and R. M. Elliott Role of the Cytoplasmic Tail Domains of Bunyamwera Orthobunyavirus Glycoproteins Gn and Gc in Virus Assembly and Morphogenesis J. Virol., September 15, 2007; 81(18): 10151 - 10160. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Altamura, A. Bertolotti-Ciarlet, J. Teigler, J. Paragas, C. S. Schmaljohn, and R. W. Doms Identification of a Novel C-Terminal Cleavage of Crimean-Congo Hemorrhagic Fever Virus PreGN That Leads to Generation of an NSM Protein J. Virol., June 15, 2007; 81(12): 6632 - 6642. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Shi, A. Kohl, V. H. J. Leonard, P. Li, A. McLees, and R. M. Elliott Requirement of the N-Terminal Region of Orthobunyavirus Nonstructural Protein NSm for Virus Assembly and Morphogenesis. J. Virol., August 1, 2006; 80(16): 8089 - 8099. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Agnihothram, J. York, and J. H. Nunberg Role of the stable signal Peptide and cytoplasmic domain of g2 in regulating intracellular transport of the junin virus envelope glycoprotein complex. J. Virol., June 1, 2006; 80(11): 5189 - 5198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Shi, K. Brauburger, and R. M. Elliott Role of N-Linked Glycans on Bunyamwera Virus Glycoproteins in Intracellular Trafficking, Protein Folding, and Virus Infectivity J. Virol., November 1, 2005; 79(21): 13725 - 13734. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. R. Novoa, G. Calderita, P. Cabezas, R. M. Elliott, and C. Risco Key Golgi Factors for Structural and Functional Maturation of Bunyamwera Virus J. Virol., September 1, 2005; 79(17): 10852 - 10863. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Briese, A. Rambaut, and W. I. Lipkin Analysis of the medium (M) segment sequence of Guaroa virus and its comparison to other orthobunyaviruses J. Gen. Virol., October 1, 2004; 85(10): 3071 - 3077. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Shi, D. F. Lappin, and R. M. Elliott Mapping the Golgi Targeting and Retention Signal of Bunyamwera Virus Glycoproteins J. Virol., October 1, 2004; 78(19): 10793 - 10802. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Shi and R. M. Elliott Analysis of N-Linked Glycosylation of Hantaan Virus Glycoproteins and the Role of Oligosaccharide Side Chains in Protein Folding and Intracellular Trafficking J. Virol., May 15, 2004; 78(10): 5414 - 5422. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kohl, R. F. Clayton, F. Weber, A. Bridgen, R. E. Randall, and R. M. Elliott Bunyamwera Virus Nonstructural Protein NSs Counteracts Interferon Regulatory Factor 3-Mediated Induction of Early Cell Death J. Virol., July 15, 2003; 77(14): 7999 - 8008. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. J. Salanueva, R. R. Novoa, P. Cabezas, C. Lopez-Iglesias, J. L. Carrascosa, R. M. Elliott, and C. Risco Polymorphism and Structural Maturation of Bunyamwera Virus in Golgi and Post-Golgi Compartments J. Virol., December 20, 2002; 77(2): 1368 - 1381. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Krauss, R. Hollinshead, M. Hollinshead, and G. L. Smith An investigation of incorporation of cellular antigens into vaccinia virus particles J. Gen. Virol., October 1, 2002; 83(10): 2347 - 2359. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kikkert, A. Verschoor, R. Kormelink, P. Rottier, and R. Goldbach Tomato Spotted Wilt Virus Glycoproteins Exhibit Trafficking and Localization Signals That Are Functional in Mammalian Cells J. Virol., January 15, 2001; 75(2): 1004 - 1012. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
