HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Gallina, A. Articles by Milanesi, G. Search for Related Content PubMed PubMed Citation Articles by Gallina, A. Articles by Milanesi, G. Agricola Articles by Gallina, A. Articles by Milanesi, G.

Intracellular retention of hepatitis B virus surface protein mutants devoid of amino-terminal pre-S1 sequences

Istituto di Genetica Biochimica ed Evoluzionistica, Consiglio Nazionale delle Ricerche, Via Abbiategrasso 207, I-27100 Pavia, Italy

To study the mechanism of L protein-mediated, intracellular (pre-Golgi) retention of hepatitis B virus (HBV) surface proteins, a collection of HBV preS-S open reading frame variants bearing wild-type or modified preS extensions was expressed in human cells. When the secretion phenotype of the corresponding proteins was analysed, all surface proteins with rearranged preS domains were found to be at least partially retained. This held true, in particular, for two variant proteins lacking preS1 amino acids 1 to 19 (ayw), the preS1 myristylated N terminus and a putative retention domain, and for another variant lacking the entire preS1 domain plus the N-terminal portion (amino acids 1 to 12) of the preS2 domain. All the retained variants underwent intracellular dimerization/oligomerization via disulphide bonds to a degree comparable to that observed in well exported natural proteins. Our results show that retention can take place in the absence of L N-terminal sequences and does not imply inhibition of covalent oligomerization.

Received 14 May 1993; accepted 30 August 1993.

This article has been cited by other articles:

				J. L. Casey, A. M. Coley, L. M. Tilley, and M. Foley Green fluorescent antibodies: novel in vitro tools Protein Eng. Des. Sel., June 1, 2000; 13(6): 445 - 452. [Abstract] [Full Text] [PDF]