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1 Institute of Biotechnology, University of Helsinki, Karvaamokuja 3A, P.O. Box 45, FIN-00014, Helsinki, Finland
and2 Institute of Chemical Physics and Biophysics, Estonian Academy of Sciences, Akadeemia 23, EE0026, Tallinn, Estonia
The complete nucleotide sequence of potato virus A (PVA) was obtained from six independent cDNA clones. The RNA genome of PVA is 9565 nucleotides long and contains one open reading frame (ORF) of 9177 bases encoding a large polyprotein of 3059 amino acids with a calculated Mr of 340K. Seven potential proteinase NIa, one HC-pro and one P1 proteinase recognition sites were found in PVA polyprotein by searching for cleavage site consensus sequences amongst the potyvirus group. The non-coding region preceding the ORF is 161 nucleotides long. The termination codon is followed by a 227-nucleotide sequence. Overall nucleotide sequence identity compared with several completely sequenced potyvirus genomes is between 53 and 58%, with overall amino acid sequence identity between 65 and 71%. When the putative amino acid sequences of individual proteins of PVA were compared with the corresponding proteins of other potyviruses, P1 and P3 appeared the least conserved (34 to 53%) whereas the other proteins were in most cases from 63 to 80% identical to each other.
Received 2 July 1993;
accepted 20 September 1993.
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