HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Okazaki, K. Articles by Kono, Y. Search for Related Content PubMed PubMed Citation Articles by Okazaki, K. Articles by Kono, Y. Agricola Articles by Okazaki, K. Articles by Kono, Y.

Expression of bovine herpesvirus 1 glycoprotein gIII by a recombinant baculovirus in insect cells

Department of Veterinary Microbiology, Faculty of Agriculture, Tokyo University of Agriculture/Technology, Fuchu, Tokyo 183, Japan

Bovine herpesvirus 1 (BHV-1) glycoprotein gIII functions both as a major virus attachment protein and haemagglutinating protein. Here we constructed recombinant baculovirus incorporating the BHV-1 gIII coding sequence to characterize the expression, function and immunogenicity of the glycoprotein in insect cells. The recombinant gIII had an M_r of 72K and seemed to form homodimers. The gIII was expressed on the surface of insect cells and a rosette formation assay demonstrated haemadsorbing activity of the glycoprotein. Antigenic authenticity of the recombinant gIII was confirmed by a panel of monoclonal antibodies specific for the glycoprotein produced in mammalian cells. Antisera raised to recombinant gIII neutralized the infectivity of BHV-1. These data suggest that recombinant gIII produced in insect cells may be a useful immunogen in a BHV-1 vaccine.

Received 5 October 1993; accepted 16 November 1993.