HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Tousch, D. Articles by Tepfer, M. Search for Related Content PubMed PubMed Citation Articles by Tousch, D. Articles by Tepfer, M. Agricola Articles by Tousch, D. Articles by Tepfer, M.

Replication of Cucumber Mosaic Virus Satellite RNA from Negative-sense Transcripts Produced Either in Vitro or in Transgenic Plants

Didier Tousch

¹ Laboratoire de Biologie Cellulaire, INRA-Centre de Versailles, F-78026 Versailles Cédex
and² Station de Pathologie Végétale, INRA-Domaine Saint-Maurice, B.P. 94, F-84143 Montfavet Cédex, France

Both positive [(+)] and negative [(-)] sense versions of two satellite RNA (satRNA) genes from cucumber mosaic virus (CMV), the necrogenic I17N and the non-necrogenic R, have been introduced into the genome of tobacco plants. On infection with satRNA-free CMV, satRNA was amplified in plants expressing each of the four genes. All four genes confer protection against CMV infection. However, co-inoculation of plants with viral RNA and CMV satRNA transcripts synthesized in vitro showed that (-) sense transcripts were less active than the corresponding (+) sense transcripts. This is the first report that (-) sense CMV satRNA transcripts can serve as a template for satRNA replication.

Present address: Laboratoire de Biochimie et Physiologie Végétales, INRA-Montpellier, 9 place Viala, F-34000 Montpellier Cédex 01, France.

Received 7 October 1993; accepted 9 December 1993.

This article has been cited by other articles:

				L.-H. Guo, Y.-H. Cao, D.-W. Li, S.-N. Niu, Z.-N. Cai, C.-G. Han, Y.-F. Zhai, and J.-L. Yu Analysis of Nucleotide Sequences and Multimeric Forms of a Novel Satellite RNA Associated with Beet Black Scorch Virus J. Virol., March 15, 2005; 79(6): 3664 - 3674. [Abstract] [Full Text] [PDF]