HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Gross, C. H. Articles by Rohrmann, G. F. Search for Related Content PubMed PubMed Citation Articles by Gross, C. H. Articles by Rohrmann, G. F. Agricola Articles by Gross, C. H. Articles by Rohrmann, G. F.

Orgyia Pseudotsugata Baculovirus p10 and Polyhedron Envelope Protein Genes: Analysis of their Relative Expression Levels and Role in Polyhedron Structure

Department of Agricultural Chemistry, Agricultural & Life Sciences 1007, Oregon State University, Corvallis, Oregon 97331-7301, U.S.A.

To investigate the regulation of p10 and polyhedron envelope protein (PEP) gene expression and their role in polyhedron development, Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis viruses lacking these genes were constructed. Recombinant viruses were produced, in which the p10 gene, the PEP gene or both genes were disrupted with the -glucuronidase (GUS) or -galactosidase (lacZ) genes. GUS activity under the control of the PEP protein promoter was observed later in infection and its maximal expression was less than 10% the level for p10 promoter-GUS constructs. Tissues from O. pseudotsugata larvae infected with these recombinants were examined by electron microscopy. Cells from insects infected with the p10^- viruses lacked p10-associated fibrillar structures, but fragments of polyhedron envelope-like structures were observed on the surface of some polyhedra. Immunogold labelling of cells infected with the p10^-GUS⁺ virus with an antibody directed against PEP showed that the PEP was concentrated at the surface of polyhedra. Although polyhedra produced by p10 and PEP gene deletion mutants demonstrated what appeared to be a polyhedron envelope by transmission electron microscopy, scanning electron microscopy showed that they had irregular, pitted surfaces that were different from wild-type polyhedra. These data suggested that both p10 and PEP are important for the proper formation of the periphery of polyhedra.

Received 8 October 1993; accepted 2 December 1993.

This article has been cited by other articles:

				L. H. L. Lua and S. Reid Virus morphogenesis of Helicoverpa armigera nucleopolyhedrovirus in Helicoverpa zea serum-free suspension culture J. Gen. Virol., October 1, 2000; 81(10): 2531 - 2543. [Abstract] [Full Text]

				Z Hu, T Luijckx, L. van Dinten, M. van Oers, J. Hajos, F. Bianchi, J. van Lent, D Zuidema, and J. Vlak Specificity of polyhedrin in the generation of baculovirus occlusion bodies J. Gen. Virol., April 1, 1999; 80(4): 1045 - 1053. [Abstract]

				J. E. Eason, R. H. Hice, J. J. Johnson, and B. A. Federici Effects of Substituting Granulin or a Granulin-Polyhedrin Chimera for Polyhedrin on Virion Occlusion and Polyhedral Morphology in Autographa californica Multinucleocapsid Nuclear Polyhedrosis Virus J. Virol., July 1, 1998; 72(7): 6237 - 6243. [Abstract] [Full Text] [PDF]

				M. H. Alaoui-Ismaili and C. D. Richardson Insect Virus Proteins (FALPE and p10) Self-Associate To Form Filaments in Infected Cells J. Virol., March 1, 1998; 72(3): 2213 - 2223. [Abstract] [Full Text] [PDF]