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J Gen Virol 75 (1994), 1189-1194; DOI 10.1099/0022-1317-75-5-1189
© 1994 Society for General Microbiology
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Detection of Bovine Viral Diarrhoea Virus p80 Protein in Subpopulations of Bovine Leukocytes

P. Sopp, L. B. Hooper, M. C. Clarke, C. J. Howard and J. Brownlie

AFRC Institute for Animal Health, Compton Laboratory, Compton, Newbury, Berkshire, RG16 0NN, U.K.

Flow cytometry and two-colour immunofluorescence were used to detect cytoplasmic bovine viral diarrhoea virus (BVDV) antigen in leukocytes from viraemic cattle. Monoclonal antibody to the p80 protein of BVDV, a non-structural viral antigen, was used to identify the subpopulations of leukocytes in which viral protein synthesis had occurred. Viral antigen was detected in 23% of peripheral blood mononuclear cells. Monocytes were found to have the highest frequency of infection (35%). A higher proportion of CD2+ T cells (23%) were infected, compared with B cells (11%) or WC1+ {gamma}{delta} T cells (11%). No significant differences in percentages of different leukocyte subpopulations in blood were detected in persistently viraemic animals compared with controls.

Received 20 August 1993; accepted 26 November 1993.


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