J Gen Virol
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J Gen Virol 75 (1994), 1259-1265; DOI 10.1099/0022-1317-75-6-1259
© 1994 Society for General Microbiology
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The analysis of polypeptides in the nuclei and cytoplasm of cells infected with murine herpesvirus 72

M. Reichel1, J. Matis1, M. Mistrikova2 and J. Lesso2

1 Institute of Virology, Slovak Academy of Sciences, Dubravska cesta 9, 842 46 Bratislava
and2 Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University, Mlynská dolina, 842 15 Bratislava, Slovakia

Twenty-six polypeptides in murine herpesvirus isolate 72 (MHV-72) were identified and the synthesis and accumulation of 21 virus-specific polypeptides in Vero cells during the course of productive infection with MHV-72 were examined. Five of the infected cell polypeptides accumulated within the nuclei and nine accumulated within the cytoplasm of MHV-72-infected cells. Seven polypeptides were identified within the nuclei and cytoplasm in equivalent amounts. The major capsid protein was shown to have an Mr of 161K. Thirteen virus-specific polypeptides were solubilized with Nonidet P-40 or radioimmunoprecipitation assay buffer and immunoprecipitated with rabbit and mouse immune sera. Analysis of the polypeptides of MHV-72 indicate a closer resemblance between MHV-72 and the gammaherpesviruses herpesvirus saimiri and Epstein—Barr virus than with the alphaherpesvirus herpes simplex virus type 1.

Received 23 September 1993; accepted 21 December 1993.


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E. Bortz, J. P. Whitelegge, Q. Jia, Z. H. Zhou, J. P. Stewart, T.-T. Wu, and R. Sun
Identification of Proteins Associated with Murine Gammaherpesvirus 68 Virions
J. Virol., December 15, 2003; 77(24): 13425 - 13432.
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