HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Luo, D. Articles by Hobom, G. Search for Related Content PubMed PubMed Citation Articles by Luo, D. Articles by Hobom, G. Agricola Articles by Luo, D. Articles by Hobom, G.

Expression and DNA binding of budgerigar fledgling disease virus large T antigen

¹ Department of Medicine
³ Department of Medical Microbiology and Infectious Disease, University of Alberta, Edmonton, Alberta, Canada T6G 2S2
and² Institut für Virologie
⁴ Institut für Mikrobiologie und Molekularbiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany

Budgerigar fledgling disease virus (BFDV) represents the first non-mammalian member of the polyomavirus genus and possesses uncommon structural and biological properties. Recombinant baculoviruses were constructed to express BFDV small t antigen, large T antigens, as well as a large T deletion mutant T_d and -galactosidase-T_d fusion proteins to high levels in infected insect cells. A recombinant virus containing a genomic copy of the BFDV early region was used for small t antigen expression, and corresponding introndeleted cDNAs for production of large T antigen derivatives. Recombinant T as well as authentic T antigen proteins from infected chicken embryo fibroblasts were purified using both immunoaffinity and DNA affinity column chromatography. We present evidence that the large T antigen interacts specifically with DNA sequences present in the non-coding region of BFDV; by indirect DNA immunoprecipitation mapping and DNase I footprinting, four regions including 12 DNA-binding sites have been determined that cover most of the BFDV non-coding region. The T antigen binding pattern observed suggests a protein-DNA interaction system considerably different from those of simian virus 40 and other polyomaviruses.

Received 23 December 1993; accepted 28 December 1993.

This article has been cited by other articles:

				L. Woolford, A. Rector, M. Van Ranst, A. Ducki, M. D. Bennett, P. K. Nicholls, K. S. Warren, R. A. Swan, G. E. Wilcox, and A. J. O'Hara A Novel Virus Detected in Papillomas and Carcinomas of the Endangered Western Barred Bandicoot (Perameles bougainville) Exhibits Genomic Features of both the Papillomaviridae and Polyomaviridae J. Virol., December 15, 2007; 81(24): 13280 - 13290. [Abstract] [Full Text] [PDF]

				R. Johne and H. Muller Polyomaviruses of Birds: Etiologic Agents of Inflammatory Diseases in a Tumor Virus Family J. Virol., November 1, 2007; 81(21): 11554 - 11559. [Full Text] [PDF]

				R. Johne, W. Wittig, D. Fernandez-de-Luco, U. Hofle, and H. Muller Characterization of two novel polyomaviruses of birds by using multiply primed rolling-circle amplification of their genomes. J. Virol., April 1, 2006; 80(7): 3523 - 3531. [Abstract] [Full Text] [PDF]

				R. Johne and H. Müller Avian polyomavirus agnoprotein 1a is incorporated into the virus particle as a fourth structural protein, VP4 J. Gen. Virol., April 1, 2001; 82(4): 909 - 918. [Abstract] [Full Text]

				R. Johne, A. Jungmann, and H. Müller Agnoprotein 1a and agnoprotein 1b of avian polyomavirus are apoptotic inducers J. Gen. Virol., May 1, 2000; 81(5): 1183 - 1190. [Abstract] [Full Text]