| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Virology, Agricultural Research Organization, The Volcani Center, PO Box 6, Bet-Dagan 50-250, Israel
and2 Pathologie Vegetale, INRA, Montfavet, France
The nucleotide sequence of the helper component protease (HC-Pro) genes of three zucchini yellow mosaic virus (ZYMV) strains has been compared with that of a helper-deficient strain of ZYMV-HC. The comparisons revealed three unique deduced amino acid differences. Two of these mutations were located in regions which are conserved in other potyviruses. The role of these mutations in aphid transmissibility was examined by exchanging DNA fragments of part of the deficient HC-Pro gene with the respective section within the gene of the infectious full-length clone of the aphid-transmissible ZYMV. The first exchange included two of the three mutations, the first coding for a change from Asp to Gly (in a non-conserved region) and the second coding for a change from Arg to Ile [within the Phe-Arg-Asp-Lys (FRNK) conserved box]. This exchange resulted in a reduced transmission (20.6% for the mutated virus compared with 57.4% in the normal ZYMV when acquired from plants and 37.2% compared with 83.1%, respectively, when acquired from membranes). The second exchange incorporated a single mutation [conferring a change from Thr to Ala within the Pro-Thr-Lys (PTK) conserved box]. This single mutation resulted in almost total loss of HC activity in aphid transmission both from plants and from membranes. The Lys residue in the conserved Lys-Ile-Thr-Cys (KITC) box, which is related to loss of HC activity in potato virus Y, tobacco vein mottling virus and in the Michigan strain of ZYMV, is unchanged in the helper-deficient ZYMV. It is therefore proposed that more than one site in HC-Pro may be functionally related to aphid transmissibility. The possible reasons for the role of these mutations in helper activity in aphid transmission of ZYMV are discussed.
Received 10 August 1993;
accepted 4 January 1994.
This article has been cited by other articles:
|
|
 |
|
  A. Dombrovsky, N. Gollop, S. Chen, N. Chejanovsky, and B. Raccah In vitro association between the helper component-proteinase of zucchini yellow mosaic virus and cuticle proteins of Myzus persicae J. Gen. Virol., May 1, 2007; 88(5): 1602 - 1610. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Stenger, B. A. Young, and R. French Random mutagenesis of wheat streak mosaic virus HC-Pro: non-infectious interfering mutations in a gene dispensable for systemic infection of plants J. Gen. Virol., September 1, 2006; 87(9): 2741 - 2747. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C. Stenger, R. French, and F. E. Gildow Complete Deletion of Wheat Streak Mosaic Virus HC-Pro: a Null Mutant Is Viable for Systemic Infection J. Virol., September 15, 2005; 79(18): 12077 - 12080. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Stenger, G. L. Hein, F. E. Gildow, K. M. Horken, and R. French Plant Virus HC-Pro Is a Determinant of Eriophyid Mite Transmission J. Virol., July 15, 2005; 79(14): 9054 - 9061. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Roudet-Tavert, S. German-Retana, T. Delaunay, B. Delecolle, T. Candresse, and O. Le Gall Interaction between potyvirus helper component-proteinase and capsid protein in infected plants J. Gen. Virol., June 1, 2002; 83(7): 1765 - 1770. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D Guo, A Merits, and M Saarma Self-association and mapping of interaction domains of helper component-proteinase of potato A potyvirus J. Gen. Virol., May 1, 1999; 80(5): 1127 - 1131. [Abstract]
|
|
|
|
|
|
J Andrejeva, U Puur, A Merits, F Rabenstein, L Jarvekulg, and J. Valkonen Potyvirus helper component-proteinase and coat protein (CP) have coordinated functions in virus--host interactions and the same CP motif affects virus transmission and accumulation J. Gen. Virol., May 1, 1999; 80(5): 1133 - 1139. [Abstract]
|
|
|
|
 |
|
 S. M. Gray and N. Banerjee Mechanisms of Arthropod Transmission of Plant and Animal Viruses Microbiol. Mol. Biol. Rev., March 1, 1999; 63(1): 128 - 148. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
