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J Gen Virol 75 (1994), 1475-1478; DOI 10.1099/0022-1317-75-6-1475
© 1994 Society for General Microbiology
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Differential transcription, without replication, of non-structural and structural genes of human parvovirus B19 in the UT7/EPO cell line as demonstrated by in situ hybridization

M. Leruez1, C. Pallier1, I. Vassias1, J. F. Elouet2, P. Romeo2 and F. Morinet1

1 Hopital Saint-Louis, Service de Microbiologie, 1 rue Claude Vellefaux, 75010 Paris
and2 Inserm U 91, Hospital Henri Mondor, Creteil, France

Erythroid progenitor cells are the main target for B19 parvovirus infection. The UT7 cell line demonstrates a marked erythroid differentiation on induction by erythropoietin (EPO) (UT7/EPO cells) and therefore appears to be a potential target for B19 parvovirus. We aimed to evaluate the presence and localization of B19 nucleic acids in UT7/EPO cells by in situ hybridization. Three digoxigenin-labelled probes were used: two recognized specifically the non-structural region of the B19 genome and one probe was structural region-specific. In our experiment UT7/EPO cells were not permissive to B19 infection. Transcription led to non-structural and structural gene transcripts without DNA replication or capsid protein synthesis.

Received 22 November 1993; accepted 9 December 1993.


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