| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Molecular Microbiology, University Medical School, Southampton General Hospital, Southampton SO9 4XY
and2 Regional Virus Laboratory, Public Health Laboratory, Myrtle Road, Bristol BS2 8EL, U.K.
Sequence comparison of the RNA-dependent RNA polymerases of small round-structured viruses (SRSVs) from 10 recent U.K. outbreaks of gastroenteritis revealed significant genetic variation. Computer analyses indicated that these viruses can be divided into two discrete groups. SRSV group I contains the previously characterized antigenic type 1 Norwalk and type 3 Southampton viruses. The amino acid sequences of the RNA polymerase, capsid and ORF3 of these two viruses are relatively similar (about 92%, 69% and 72% amino acid identity, respectively). A representative member of group II SRSVs, Bristol virus, was subjected to a detailed genetic analysis. Bristol virus is a recent antigenic type 2 isolate from a U.K. hospital outbreak of gastroenteritis. Using a single clinical sample the 3'-terminal 3881 nucleotide cDNA sequence [excluding the poly(A) tail] of this virus was determined. Analysis of the sequence revealed significant differences from those of group I viruses with the RNA polymerase region, capsid and ORF3 showing only about 62%, 43% and 30% amino acid identity respectively with the equivalent proteins of the Norwalk and Southampton viruses. These data suggest that the morphologically identical SRSVs belong to at least two genetically distinct groups.
Received 10 December 1993;
accepted 8 February 1994.
This article has been cited by other articles:
|
|
 |
|
  N. Iritani, H. Vennema, J. J. Siebenga, R. J. Siezen, B. Renckens, Y. Seto, A. Kaida, and M. Koopmans Genetic Analysis of the Capsid Gene of Genotype GII.2 Noroviruses J. Virol., August 1, 2008; 82(15): 7336 - 7345. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Tsugawa, K. Numata-Kinoshita, S. Honma, S. Nakata, M. Tatsumi, Y. Sakai, K. Natori, N. Takeda, S. Kobayashi, and H. Tsutsumi Virological, Serological, and Clinical Features of an Outbreak of Acute Gastroenteritis Due to Recombinant Genogroup II Norovirus in an Infant Home J. Clin. Microbiol., January 1, 2006; 44(1): 177 - 182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Han, Q. Wang, J. R. Smiley, K. O. Chang, and L. J. Saif Self-Assembly of the Recombinant Capsid Protein of a Bovine Norovirus (BoNV) into Virus-Like Particles and Evaluation of Cross-Reactivity of BoNV with Human Noroviruses J. Clin. Microbiol., February 1, 2005; 43(2): 778 - 785. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Han, J. R. Smiley, C. Thomas, and L. J. Saif Genetic Recombination between Two Genotypes of Genogroup III Bovine Noroviruses (BoNVs) and Capsid Sequence Diversity among BoNVs and Nebraska-Like Bovine Enteric Caliciviruses J. Clin. Microbiol., November 1, 2004; 42(11): 5214 - 5224. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Dingle Mutation in a Lordsdale Norovirus Epidemic Strain as a Potential Indicator of Transmission Routes J. Clin. Microbiol., September 1, 2004; 42(9): 3950 - 3957. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Haramoto, H. Katayama, and S. Ohgaki Detection of Noroviruses in Tap Water in Japan by Means of a New Method for Concentrating Enteric Viruses in Large Volumes of Freshwater Appl. Envir. Microbiol., April 1, 2004; 70(4): 2154 - 2160. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. C. Shieh, R. S. Baric, J. W. Woods, and K. R. Calci Molecular Surveillance of Enterovirus and Norwalk-Like Virus in Oysters Relocated to a Municipal-Sewage-Impacted Gulf Estuary Appl. Envir. Microbiol., December 1, 2003; 69(12): 7130 - 7136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nishida, H. Kimura, M. Saitoh, M. Shinohara, M. Kato, S. Fukuda, T. Munemura, T. Mikami, A. Kawamoto, M. Akiyama, et al. Detection, Quantitation, and Phylogenetic Analysis of Noroviruses in Japanese Oysters Appl. Envir. Microbiol., October 1, 2003; 69(10): 5782 - 5786. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Oehmig, M. Buttner, F. Weiland, W. Werz, K. Bergemann, and E. Pfaff Identification of a calicivirus isolate of unknown origin J. Gen. Virol., October 1, 2003; 84(10): 2837 - 2845. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kageyama, S. Kojima, M. Shinohara, K. Uchida, S. Fukushi, F. B. Hoshino, N. Takeda, and K. Katayama Broadly Reactive and Highly Sensitive Assay for Norwalk-Like Viruses Based on Real-Time Quantitative Reverse Transcription-PCR J. Clin. Microbiol., April 1, 2003; 41(4): 1548 - 1557. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. H. Johansson, M. Torven, A.-C. Hammarlund, U. Bjorne, K.-O. Hedlund, and L. Svensson Food-Borne Outbreak of Gastroenteritis Associated with Genogroup I Calicivirus J. Clin. Microbiol., March 1, 2002; 40(3): 794 - 798. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Pfister and E. Wimmer Polypeptide p41 of a Norwalk-Like Virus Is a Nucleic Acid-Independent Nucleoside Triphosphatase J. Virol., February 15, 2001; 75(4): 1611 - 1619. [Abstract] [Full Text]
|
|
|
|
|
|
J. Vinjé and M. P. G. Koopmans Simultaneous Detection and Genotyping of "Norwalk-Like Viruses" by Oligonucleotide Array in a Reverse Line Blot Hybridization Format J. Clin. Microbiol., July 1, 2000; 38(7): 2595 - 2601. [Abstract] [Full Text]
|
|
|
|
|
|
F Roerink, M Hashimoto, Y Tohya, and M Mochizuki Organization of the canine calicivirus genome from the RNA polymerase gene to the poly(A) tail J. Gen. Virol., April 1, 1999; 80(4): 929 - 935. [Abstract]
|
|
|
|
|
|
A. J. Maguire, J. Green, D. W. G. Brown, U. Desselberger, and J. J. Gray Molecular Epidemiology of Outbreaks of Gastroenteritis Associated with Small Round-Structured Viruses in East Anglia, United Kingdom, During the 1996-1997 Season J. Clin. Microbiol., January 1, 1999; 37(1): 81 - 89. [Abstract] [Full Text]
|
|
|
|
|
|
J. Green, K. Henshilwood, C. I. Gallimore, D. W. G. Brown, and D. N. Lees A Nested Reverse Transcriptase PCR Assay for Detection of Small Round-Structured Viruses in Environmentally Contaminated Molluscan Shellfish Appl. Envir. Microbiol., March 1, 1998; 64(3): 858 - 863. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
